LINC01060对人胃癌BGC-823细胞系生物学行为的作用研究

The effect of LINC01060 on biological behavior of human gastric cancer BGC-823 cell line

目的:探索LINC01060对人胃癌BGC-823细胞系生物学行为的作用。方法:生信数据分析LINC01060在胃癌中的表达情况,建立LINC01060过表达和沉默的稳转BGC-823细胞株,Real time PCR验证LINC01060的表达,CCK-8检测细胞增殖,流式细胞术检测细胞凋亡,划痕实验检测细胞迁移,Transwell检测细胞侵袭,Western blot检测EMT相关蛋白E-cadherin、Vimentin、N-cadherin和Snail蛋白的表达。结果:GEO数据库发现,LINC01060在胃癌中低表达(P<0.05);Real time PCR结果显示,沉默组LINC01060的表达显著下调(P<0.05),过表达组LINC01060的表达显著上调(P<0.05);CCK-8显示,过表达组细胞增殖能力显著下降(P<0.05);流式细胞术显示,过表达组细胞凋亡比例显著上升(P<0.05);Transwell结果显示,过表达组细胞侵袭能力显著下降(P<0.05);细胞划痕显示,过表达组细胞迁移能力显著下降(P<0.05);Western blot显示,过表达组E-cadherin蛋白表达水平显著上调(P<0.05),Vimentin、N-cadherin、Snail蛋白表达水平显著下调(P<0.05);以上实验沉默组均相反。结论:LINC01060在人胃癌BGC-823细胞中发挥促进凋亡,抑制增殖、侵袭、迁移和EMT的作用。

Objective:To explore the effect of LINC01060 on biological behavior of human gastric cancer BGC-823 cell line.Methods:The expression of LINC01060 in gastric cancer was analyzed by GEO database, and the stable BGC-823 cell line with overexpression and silencing of LINC01060 was established.The expression of LINC01060 was verified by Real time PCR.Cell proliferation was detected by CCK-8 assay.Cell apoptosis was detected by flow cytometry.Cell metastasis was detected by scratch assay.Cell invasion was detected by Transwell assay, and the expressions of EMT-related proteins E-cadherin, Vimentin, N-cadherin and Snail were detected by Western blot.Results:The GEO database found that the expression of LINC01060 was low in gastric cancer(P<0.05).Real time PCR results showed that the expression of LINC01060 in the silence group was significantly down-regulated(P<0.05),and the expression of LINC01060 in the over-exposure group was significantly up-regulated(P<0.05).CCK-8 showed that the proliferation ability of cells in the overexpression group was significantly decreased(P<0.05).Flow cytometry showed that the proportion of cells apoptosis in the overexpression group was significantly increased(P<0.05).Transwell results showed that the cell invasion ability of the overexpression group was significantly decreased(P<0.05).Cell scratch showed that the cell migration ability of the overexpression group was significantly decreased(P<0.05).Western blot showed that the expression level of E-cadherin protein in the overexpression group was significantly up-regulated(P<0.05),and Vimentin, N-cadherin, Snail protein expression level was significantly down-regulated(P<0.05).The above experiments were the opposite in silence group.Conclusion:LINC01060 can promote apoptosis and inhibit proliferation, invasion, migration and EMT in human gastric cancer BGC-823 cell line.