A BIOSENSOR USING COUPLED PLASMON WAVEGUIDE RESONANCE COMBINED WITH HYPERSPECTRAL FLUORESCENCE ANALYSIS

We developed a biosensor that is capable for simultaneous surface plasmon resonance(SPR)sensing and hyperspectral fuores cence analysis in this paper.A symmetrical metal-dielectric slabscheme is employed for the excitation of coupled plasnon waveguide resonance(CPWR)in thepresent work.Resonance bet ween surface plasmon mode and the guided waveguide mode gen-erates narrower full width half-maximum of the refective curves which leads to increased pre.cision for the determination of refractive index over conventional SPR sensors.In addition,CPWR also fers longer surface propagation depths and higher surface electric field strengthsthat enable the excitation of fluorescence with hyperspectral technique to maintain an appreci-able signal-to-noise ratio.The refractive index information obtained from SPR sensing and thechemical properties obt ained through hyperspectral fluorescence analysis confirm each other toexclude false-positive or false-negative cases.The sensor provides a comprehensive understandingof the biological events on the sensor chips.