太赫兹刺激听神经的测试平台搭建

The Establishment of Test Platforms for Terahertz Stimulation of Auditory Nerve

目的近年来,用于脑功能调控的神经调控技术蓬勃发展,很多方法已在临床上被推广应用,主要包括电极深部脑刺激、经颅磁刺激、光遗传技术、超声深脑刺激等。但是这些调控技术存在刺激靶点改变灵活性差、空间分辨率不足、需要注射病毒转染等问题。与这些技术相比,太赫兹波调控则能以较高的时空分辨率、无需引入外源基因的方式对神经活动进行干预。激光神经刺激是一种具有较明确靶向性的刺激方法,可以通过调整不同激光参数(激光波长、脉冲能量等)控制引起神经兴奋或者抑制。但是由于该研究方向的实验手段和实验平台的缺乏,相关研究开展较少。方法针对这个问题,从听觉神经入手,在分子、细胞和在体不同层面为相关领域的研究搭建了不同的测试平台。结果实验结果表明,这些系统在时间和空间上具有良好的耦合性和靶向性,测得的信号受噪音干扰小。结论这些系统可以有效测试神经系统对太赫兹刺激的响应并精确控制刺激时间和位置。

Objective In recent years,neuromodulation techniques for brain function regulation have developed vigorously,and many methods have been popularized and applied in clinical practice,mainly including electrode deep brain stimulation,transcranial magnetic stimulation,photogenetic technology,ultrasonic deep brain stimulation,etc.However,these regulatory techniques have some problems,such as poor flexibility of stimulating target change,insufficient spatial resolution,and need to inject virus transfection.Compared with these techniques,terahertz(THz)wave regulation can intervene neural activity with higher spatial and temporal resolution without introducing foreign genes.Laser nerve stimulation is a specifically targeted stimulation method,which can control the excitation or inhibition of nerve by adjusting different laser parameters(laser wavelength,pulse energy,etc.).However,due to the lack of experimental means and platforms in this research direction,few relevant studies have been carried out.Methods To sum up,this paper sets up different testing platforms for related researches at molecular,cellular and somatic levels,starting from auditory nerve.Results The experimental results show that these systems have good coupling and targeting properties in time and space,and the measured signals are less disturbed by noise.Conclusion These systems can effectively test the response to terahertz stimulation and precisely control the timing and location of the stimulus.