84消毒液重复染毒对大鼠毒性试验研究

Study on rats toxicity effect of 84 disinfectant repeated exposure

目的研究SD大鼠反复暴露于84消毒液对多种器官的伤害情况。方法SD大鼠30只,雌雄各半,在密闭空间内分别按100 mL/m^(2)喷洒含0(对照组)、400、10000 mg/L次氯酸钠的84消毒液,大鼠暴露2次/d,每次1 h,持续28 d,腹主动脉穿刺收集血样,用于血液学和血液生化指标检测,于第29天,所有大鼠麻醉后实施安乐死,采集肝、肾、心、脾、肺、气管、眼球和皮肤等重要脏器进行进一步检查。结果高剂量组大鼠重复染毒28 d后,气管、肺、肝、肾、血液系统出现病变,眼和呼吸道出现急性刺激症状,血细胞增多和凝血功能障碍,气管、肺、肝和肾发生病理学改变。低剂量组和对照组无异常改变。结论长期大剂量接触84消毒液可能对动物机体健康造成不良影响,在使用中应加强个人防护。

Objective To evaluate the toxicity of Sprague Dawley rats after repeated exposure to 84 disinfectant.Methods Thirty SD rats were divided randomly into control,low and high dose group,ten rats for each group.Rats were exposed in 100 mL/m^(3) room sprayed with 0,400 and 10000 mg/L sodinm hypochlorite respectively.The rats were exposed two times each day,1 hour each time,for 28 days.Then,blood,clinical symptoms,hematological,biochemical and histopathological examinations were performed.Results Rats developed a series of lesions in the tracheas,lung,liver,kidney and blood after exposure for 28 days.Acute irritation symptoms were observed in respiratory and eyes.Hematotoxicity was evidenced by thrombocytes changes and coagulation dysfunction.Histopathological alterations were found in the tracheas,lungs,live and kidney.Conclusion Therefore,the present study suggested that long-term exposure to high-dose of 84 disinfectant may cause unneglectable adverse effects to human health and highlighted the importance of personal protection against sodium hypochlorite disinfectant.