芋头挥发性代谢物分析及其品质综合评价

Volatiles Metabolites Analysis and Evaluation on Quality Traits of Different Tora Varieties

芋头是一种重要的蔬菜兼粮食作物,其营养丰富且药用价值高,然而对于芋头品质性状的评价及分子机理的研究较少。以来源于我国不同地区的18个芋头品种为例,运用生物信息学方法系统评价其外观和营养物质指标,并利用GC-MS气质联用法对挥发性代谢物进行定性和定量分析。结果表明,魁芋芋头的单芋重、干物质和淀粉含量相对高于多子芋。不同芋头品种的品质指标存在明显差异,主成分分析降维方法分析到了4个独立的综合指标,其累计贡献率达82.31%,解释了芋头品质的大部分变异信息。综合得分排名前5名的全部是魁芋。以炭步香芋和花都红芽芋为例的代谢组学分析,共鉴定到76种挥发性代谢物,其中香型中显著上调的代谢物包括1-甲基乙基-萘和4-甲基-6-庚烯-4油化物,说明这2种代谢物很可能与香味生物学功能有关。

Taro is an important vegetable as well as a staple crop, which is abundant in nutrition and drugs components. However, the studies on the evaluation of taro quality traits and its molecular mechanism were less reported. We systematically evaluated the appearance and nutrient indexes of 18 taro varieties derived from different ecological regions of China by bioinformatics methods, and quantitively and qualitatively determined the relative abundance of volatiles metabolites with GC-MS approach. The results showed that weight per taro,dry mass and starch contents were relatively higher in Kui taro than that in Duozi taro. The quality traits in different taro varieties exert huge variation. Principal component analysis(PCA) revealed the four independent indexes from the determined quality traits, with 82.31% accumulative contribution rates, which reflected the majority variation information. The top five comprehensive scores were found to belong to Kui taro.Furthermore, we analyzed the differentially abundant volatiles metabolites in Tanbu xiang taro and Huaduhongya taro, and identified 76 volatiles metabolites, including two extremely upregulated metabolites, 4-methyl-6-hepten-4-olide and 1-methylethyl-naphthalene, especially in Tanbuxiang taro. The two key metabolites are very likely related to the biological function of aromatic profiles.