生活污水短程硝化脱氮工艺的快速启动及稳定性研究

RAPID START-UP AND STABILITY OF PARTIAL NITRIFICATION FOR DOMESTIC SEWAGE

以低碳氮比的生活污水为研究对象,采用SBR反应器,通过减少好氧阶段的搅拌时间快速启动短程硝化脱氮过程,对典型运行周期内氮去除规律进行研究,并从微生物角度进一步验证了短程硝化脱氮工艺的实现。结果表明:减少50%好氧搅拌时间后,亚硝酸盐积累率(NAR)由36.05%增加到54.06%,好氧阶段停止搅拌后,NAR被提高到90.17%,并且以此状态持续稳定运行;典型运行周期内SBR具有良好的NH^(+)_(4)-N去除效果和较高的NAR,实测NH^(+)_(4)-N去除率达89.46%,出水NAR达89.13%;实时荧光定量PCR技术(q-PCR)检测表明,经过140 d的种群优化,污泥中氨氧化菌(AOB)和亚硝酸盐氧化菌(NOB)含量分别占总菌数的70.3%和2.1%,从分子生物学角度验证了短程硝化工艺的实现。

In this paper,domestic sewage with low carbon to nitrogen ratio was taken as the research object,and the SBR reactor was used to quickly start the partial nitrification by reducing the stirring time in aerobic stage.The nitrogen removal law of typical operating cycles was studied,and the realization of partial nitrification was further verified from the perspective of microorganisms.After reducing the aerobic stirring time by 50%,the nitrite accumulation rate(NAR) increased from 36.05% to 54.06%.After stopping aerobic stirring,the NAR increased to 90.17%,and it continued to operate stably in this state.In a typical operation cycle,SBR had a good ammonia nitrogen removal effect and high NAR.The removal rate of NH^(+)_(4)-N reached 89.46% and the NAR of effluent reached 89.13%.The detection result of quantitative real-time PCR(q-PCR) showed that after 140 days of population optimization,the contents of ammonia oxidizing bacteria(AOB) and nitrite oxidizing bacteria(NOB) in the sludge accounted for 70.3% and 2.1% of the total bacteria,respectively.The realization of partial nitrification was verified from the perspective of molecular biology.