CO_(2)浓度对微藻-真菌共生体生物净化沼液沼气的影响

EFFECT OF CO_(2) CONCENTRATION ON SIMULTANEOUS PURIFICATION OF BIOGAS SLURRY AND BIOGAS BY MICROALGAE-FUNGI CO-CULTURE TECHNOLOGY

以微藻-真菌(小球藻-灵芝菌)共生体系为研究对象,并在2种浓度(10^(-7),10^(-9)mol/L)合成独角金内酯(GR24)的诱导下,探究6种CO_(2)浓度对该体系同步净化沼液和沼气的影响。在GR24的诱导下,微藻-真菌系统的代谢和微藻的光合作用增强,使藻-菌共生体快速生长,进一步增强了系统的净化性能。此外,GR24通过提高微藻细胞碳酸酐酶活性,增强了共培养体系的CO_(2)去除性能。结果表明:最佳的GR24浓度为10^(-9)mol/L,最适宜藻-菌共生体系的CO_(2)浓度为45%,在此最优条件下,小球藻-灵芝菌共生体系对沼液的COD、TN和TP的平均去除率分别为(83.37±8.04)%、(82.07±7.74)%和(85.43±8.26)%,沼气中CO_(2)的平均去除率为(62.07±5.94)%。

In this paper,the microalgae-fungus(Chlorella-Ganoderma) symbiotic system was chosen as the research object,and the effects of different CO_(2) concentrations on simultaneous purification of biogas slurry and biogas were investigated under the induction of two concentrations of synthetic unicolactone(GR24,10^(-7),10^(-9) mol/L).Under the induction of GR24,the metabolism and photosynthesis of microalgae in the microalgae-fungal system were enhanced,which made the algal-bacterial symbiosis grow rapidly and further enhanced the purification performance of the system.In addition,GR24 enhanced CO_(2) removal performance of the co-culture system by increasing the activity of carbonic anhydrase in microalgae cells.The results showed that the optimal GR24 concentration was 10^(-9) mol/L,and the CO_(2) concentration was 45%.Under these optimal conditions,the average removal rates of COD,TN and TP were(83.37±8.04)%,(82.07±7.74)% and(85.43±8.26)%,respectively,and the average removal rate of CO_(2) in biogas was(62.07±5.94)%.