稻曲病菌有性生殖中光诱导基因的转录组分析

Transcriptomic analysis of light-induced sexual reproduction in Villosiclava virens

稻曲病菌(Villosiclava virens)能够以菌核的形式安全越冬并在来年萌发产生子囊孢子,成为稻曲病菌的重要初侵染源;而菌核产生子实体的过程需要光的诱导。为了探索光诱导的分子机制,本文对不同光照处理条件下稻曲病菌菌核萌发过程的转录组学进行了比较分析。结果发现,与黑暗条件下菌核的萌发相比,光照处理后542个基因显著差异表达,其中上调359个,下调183个。GO富集分析发现,DEGs显著富集于糖基水解酶活性、细胞壁生物合成、氧化还原酶活性等。KEGG分析发现,DEGs显著富集于脂质代谢、氨基酸代谢、次生代谢产物合成等代谢通路。DEGs中子实体独有基因组氨酸激酶为光感受器,其响应光信号引起下游基因的表达,同时,与有性生殖过程相关的水通道蛋白和甲基转移酶蛋白编码基因表达活跃,共同调控了菌核萌发产生子实体过程。

Sclerotia are the main overwintering structures in Villosiclava virens,and they germinate to produce ascospores in the coming year,which are the primary infection source.In order to explore the molecular mechanism of sclerotia germination induced by light,sclerotia were cultured under dark and light conditions respectively,and sampled for high-throughput sequencing.Combined with gene differential expression analysis and function annotation,the key genes of sclerotia germination in V.virens were identified.The differentially expressed genes were verified by qRT-PCR.Data analysis showed that 542 genes were significantly differentially expressed by light treatment,of which 359 and 183 genes were up-and down-regulated,respectively.GO enrichment analysis showed that the DEGs were significantly enriched in glycosylhydrolase activity,cell wall biosynthesis and oxidoreductase activity,etc.KEGG analysis showed that the DEGs were significantly enriched in metabolic pathways such as lipid metabolism,amino acid metabolism,and secondary metabolite synthesis.The histidine kinase gene unique to the fruiting body is a photoreceptor,which responds to light and induces the expression of downstream genes.Meanwhile,the aquaporin and methyltransferase genes related to sexual reproduction are actively expressed.It is speculated that these genes promoted the process of sclerotia germination and fruiting body formation.