侵染云南鸡蛋果的病毒种类鉴定及CMV cp基因序列分析

Identification of the viruses infecting Passiflora edulis in Yunnan and sequence analysis of the cp gene of CMV infecting P.edulis

鸡蛋果(Passiflora edulis)别名百香果、紫果西番莲,为西番莲科(Passifloraceae)西番莲属(Passiflora)多年生草质藤本植物,原产于安的列斯群岛^([1])。鸡蛋果营养价值高、风味独特,是一种天然饮料作物。云南独特的地理气候条件,适宜鸡蛋果种植。1990年西双版纳开始集约化种植鸡蛋果(现大多称为百香果)^([2])。近年来鸡蛋果产业在云南发展迅速,但病毒病和根腐病等病害逐渐成为鸡蛋果产业发展的限制因素之一^([2])。

Viral diseased Passiflora edulis samples showing mosaic,shrinking,chlorosis,and malformation were collected from Yuxi,Wenshan,Dehong,and Xishuangbanna in Yunnan Province.Forty diseased samples were detected by RT-PCR/PCR using degenerate primers of members in genera of Umbravirus,Tobamovirus,Luteovirus,Orthotospovirus,Begomovirus,Badnavirus,Potyvirus and specific primers of cucumber mosaic virus(CMV).Results showed that CMV,telosma mosaic virus(TeMV)and papaya leaf curl Guangdong virus(PaLCuGdV)were detected in the diseased P.edulis samples.CMV showed the highest detection rate of 27.5%,while TeMV and PaLCuGdV had the rates of 20.0%and 7.5%respectively among the P.edulis samples.Mixed infections of PaLCuGdV+CMV,PaLCuGdV+TeMV and CMV+TeMV were also found in these samples.The 657 nucleotide(nt)CMV full-length coat protein(CP)gene was cloned and sequenced from two diseased samples in Yuxi(YYXi-JDG,Acc.No.MW495062)and Xishuangbanna(YXSBN-JDG,Acc.No.MW495063),respectively.These two CMV isolates shared 97.3%identity of nt sequence with each other,while had 76.9%-97.7%and 77.0%-98.2%nt identity with other CMV isolates,respectively.Phylogenetic tree showed that the two CMV isolates from P.edulis belonged to CMV subgroupⅠ,and no obvious geographic differentiation and host specificity relationship was found among the selected 33 CMV isolates worldwide.