宫颈癌组织中MiR-1287-5p、HOXA7表达及临床意义

Expressions and clinical significances of MiR-1287-5p and homeobox A7 in cervical cancer tissues of patients

目的:探究miR-1287-5p、同源盒基因A7(HOXA7)在宫颈癌组织中的表达及临床意义。方法:收集2013年6月-2017年1月本院收治的宫颈癌患者92例临床资料,qRT-PCR法检测宫颈癌组织和癌旁组织中MiR-1287-5p和HOXA7mRNA表达,Pearson分析MiR-1287-5p与HOXA7表达相关性;Kaplan-Meier生存曲线分析MiR-1287-5p和HOXA7表达水平与宫颈癌患者预后关系;COX回归分析影响宫颈癌患者预后的危险因素。结果:宫颈癌组织中miR-1287-5p(0.71±0.16)低于癌旁组织(1.03±0.05),HOXA7 mRNA(1.51±0.24)高于癌旁组织(1.02±0.03)(均P<0.05)宫颈癌组织中MiR-1287-5p与HOXA7表达呈负相关(r=-0.484,P<0.05);MiR-1287-5p和HOXA7表达水平与患者人乳头瘤病毒(HPV)感染、淋巴结转移、分化状态和FIGO分期有关(P<0.05);Kaplan-Meier分析显示,MiR-1287-5p高表达组生存率高于低表达组(logrank=12.471,P=0.000),HOXA7低表达组生存率高于高表达组(logrank=5.180,P=0.023);多因素COX回归分析,HPV感染阳性、组织低分化状态、FIGO分期Ⅲ-Ⅳ期、miR-1287-5p低表达和HOXA7高表达是影响宫颈癌患者预后不良的独立危险因素(P<0.05)。结论:宫颈癌组织中miR-1287-5p表达降低,HOXA7表达升高,二者异常表达影响宫颈癌患者预后。

Objective: To explore the expressions and clinical significance of miR-1287-5p and homeobox A7(HOXA7) in cervical cancer of patients. Methods: The clinical data of 92 patients with cervical cancer from June 2013 to January 2017 were collected in this study. The expression of miR-1287-5p and HOXA7 in cervical cancer tissue and in paracancerous tissue were detected by qRT-PCR. Pearson analysis was applied to analyze the correlation between the MiR-1287-5p level in cervical cancer tissues of the patients and their HOXA7 expression level. Kaplan-Meier survival curves were applied to analyze the correlation between the MiR-1287-5p and HOXA7 expression levels of the patients with cervical cancer and their prognosis. COX regression was applied to analyze the risk factors affecting the prognosis of the patients with cervical cancer. Results: The miR-1287-5p level(0.71±0.16) in cervical cancer tissue was significantly lower than that(1.03±0.05) in paracarcinoma tissue, and the HOXA7 mRNA level(1.51±0.24) was significantly higher than that(1.02±0.03) in paracarcinoma tissue(all P<0.05). The miR-1287-5p level in cervical cancer tissue was negatively correlated with the HOXA7 level(r=-0.484, P<0.05). The MiR-1287-5p and HOXA7 expression levels in cervical cancer tissue of the patients were associated with their human papillomavirus(HPV) infection, their lymph node metastasis, their differentiation status of cancer, and their FIGO staging(P<0.05). Kaplan-Meier analysis showed that the survival rate of the patients with high MiR-1287-5p expression level was significantly higher than that of the patients with low Mir-1287-5p expression level(logrank=12.471, P=0.000), and the survival rate of the patients with low HOXA7 expression level was significantly higher than that of the patients with high Mir-1287-5p expression level(logrank=5.180, P=0.023). Multivariate COX regression analysis showed that the positive HPV infection, the low tissue differentiation of cancer, the FIGO stage Ⅲ-Ⅳ of cancer, the low expression level of Mir-887-5p,and the high expression level of HOXA7of the patients with cervical cancer were the independent risk factors of their poor prognosis(P<0.05).Conclusion:The expression level of miR-1287-5p in cancer tissues of the patients decreases,and the expression level of HOXA7in cancer tissues of the patients increases,and the abnormal expression levels of both which influence the prognosis of the patients with cervical cancer.