机械振动对去卵巢后骨质疏松骨折大鼠雌激素和脑源性神经营养因子表达的影响

The effects of mechanical vibration on the expression of estrogen and brain-derived neurotrophic factor after ovariectomy

目的探讨机械振动对去卵巢后骨质疏松骨折大鼠海马雌激素和骨折端脑源性神经营养因子(BDNF)表达的影响。方法选取3月龄雌性Wistar大鼠30只,按照随机数字表法将其分为对照组、去卵巢组、振动组,每组10只。对照组建立骨折模型,去卵巢组和振动组建立去卵巢后骨质疏松骨折模型,采用频率35 Hz、每日20 min、每周5 d的全身垂直振动作用于振动组。干预2周和6周后,采用X线评价各组大鼠的骨折愈合情况,并通过酶联免疫吸附实验和免疫印记法分别检测大鼠海马雌激素、骨折端BDNF的含量。结果干预2周和6周后,振动组大鼠骨折愈合率较对照组和去卵巢组高(P<0.05)。振动组大鼠干预2周和6周后的海马雌激素含量[(0.72±0.03)ng/ml、(1.19±0.03)ng/ml]较对照组和去卵巢组高(P<0.05),干预2周和6周后的骨折端BDNF含量[(0.26±0.01)ng/ml、(0.39±0.06)ng/ml]较对照组低、较去卵巢组高(P<0.05)。大鼠骨折端BDNF表达水平与其骨折愈合率高度相关。结论机械振动可以促进大鼠海马雌激素和骨折端BDNF表达,加速去卵巢后骨质疏松大鼠骨折愈合。

Objective To explore any effect of mechanical vibration on the expression of estrogen and brain-derived neurotrophic factor(BDNF)in ovariectomized rats with an osteoporotic fracture.Methods Thirty 3-month-old female Wistar rats were divided randomly into a control group,an ovariectomy group and a vibration group,each of 10.Fractures were induced in the rats of all three groups.Twenty minutes of whole-body vertical vibration was applied to the vibration group at a frequency of 35Hz,5 days a week for 6 weeks.After 2 and 6 weeks the fracture healing of each group was evaluated using X-rays,the levels of hippocampal estrogen were measured using enzyme-linked immunosorbent assays and fracture-end BDNF was quantified by immunoblotting.Results After 2 and 6 weeks of vibration the average fracture healing in the vibration group was significantly greater than in the other 2 groups.The average estrogen content in the hippocampus of the vibration group was significantly higher than in the other 2 groups after both 2 and 6 weeks,while the average BDNF content in their fracture ends was significantly lower.The BDNF expression at the fracture end was significantly correlated with the fracture healing.Conclusion Mechanical vibration can promote the expression of estrogen and BDNF in the hippocampus and accelerate fracture healing in osteoporotic rats.