新型冠状病毒ORF3a蛋白质的生物信息学分析

Bioinformatics analysis of ORF3a protein of SARS-CoV-2

目的用生物信息学分析严重急性呼吸综合征冠状病毒2(SARS-CoV-2)基因ORF3a蛋白质。方法从UniProt数据库获取ORF3a蛋白质的氨基酸序列,应用ProtParam tool分析其理化性质,SOPMA、SWISS-MODEL预测二、三级结构,PortScale、Signal IP 4.0 Server、TMHMM Server 2.0分析ORF3a蛋白质亲疏水性、信号肽、跨膜区。应用ABCpred、SYFPEITHI和NetPhos 3.1 Server预测B、T细胞表面抗原表位及磷酸化位点,VaxiJenv 2.0、AlgPred server预测抗原性和过敏原性。结果ORF3a蛋白质含275个氨基酸,为稳定、疏水性蛋白质,有3个跨膜螺旋区、29个磷酸化位点,含多个T、B细胞抗原表位,具有抗原性和非过敏原性。结论ORF3a蛋白质结构稳定,含多个T、B细胞抗原表位,可为研究SARS-CoV-2多表位疫苗提供基础。

Objective To analyze ORF3a protein in severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)by bioinformatics.Methods Amino acid sequence of ORF3a was obtained from UniProt.Physicochemical properties of the protein were analyzed by ProtParam tool.Secondary and tertiary structures were predicted by SOPMA and SWISS-MODEL.Hydrophobicity,signal peptide,and transmembrane region of ORF3a protein were analyzed by PortScale,Signal IP 4.0 Server,and TMHMM Server 2.0.B cell and T cell surface epitopes as well as phosphorylation sites were predicted by ABCpred,SYFPEITHI and NetPHos 3.1 Server.Antigenicity and allergenicity were predicted by VaxiJenv 2.0 and AlgPred server.Results ORF3a is a stable,hydrophobic protein with 3 transmembrane helical regions and 29 phosphorylation sites.It contains 275 amino acids and multiple T and B cell epitopes,and is antigenic and non-allergenic.Conclusion ORF3a protein is structurally stable.It contains multiple T and B cell epitopes,thus can provide basis for the study of SARS-CoV-2 multi-epitope vaccines.