rhTPO对异基因造血干细胞移植模型造血重建的作用

Effect of Recombinant Human Thrombopoietin(rhTPO) on Hematopoietic Reconstitution in Allogeneic Hematopoietic Stem Cell Transplantation Model

目的:探讨重组人血小板生成素(rh TPO)对异基因造血干细胞移植模型造血重建的作用。方法:以C57BL/6小鼠为供鼠,BALB/c小鼠为受鼠,分离供鼠骨髓单个核细胞,预处理后,给予受鼠尾静脉注射供鼠骨髓单个核细胞5×10^(6)/只,建立异基因造血干细胞移植模型,移植后d 28用流式细胞术检测受鼠骨髓造血干细胞植入情况。将建模成功后的受鼠随机分为两组,实验组于移植后d 1开始注射rh TPO,对照组注射生理盐水,两组均连续注射14 d。移植后d 1、3、7、14、21观察两组受鼠外周血及骨髓造血情况。结果:移植后28 d,受鼠骨髓H-2Kb表达率为43.85%(>20%),受鼠嵌合成功。移植后d 3、7、14、21,实验组受鼠血小板数高于对照组,比较差异具有统计学意义(P<0.05)。两组小鼠在移植后d 1、3、7均出现骨髓造血功能抑制,但实验组骨髓造血增生较对照组好。移植后d 14、21,两组小鼠骨髓造血功能均较前有所恢复,且实验组恢复明显。结论:rh TPO能有效刺激异基因造血干细胞移植后血小板生成,并促进移植后骨髓造血恢复及造血重建。

Objective:To explore the effect of recombinant human thrombopoietin (rh TPO) on hematopoietic reconstruction in allogeneic hematopoietic stem cell transplantation (allo-HSCT) model.Methods:The C57BL/6 mice were employed as the donors,and BALB/c mice as recipients.The bone marrow mononuclear cells of the donor mice were extracted and pretreated,which then were injected with 5×10^(6) per mouse through the tail vein of the recipient to establish an allo-HSCT model.The implantation of hematopoietic stem cells in the recipient mice was detected by flow cytometry on the 28th day after transplantation.Next,the successfully modeled recipient mice were randomly divided into experimental group and control group.The rh TPO was injected into mice in the experimental group on the first day after transplantation,while the saline was injected into mice in the control group.Both groups were injected for 14 consecutive days.The peripheral blood and bone marrow hematopoiesis of the two groups were observed on day 1,3,7,14,and 21after transplantation.Results:The expression rate of H-2Kb in the bone marrow of recipient mice was 43.85%(>20%) on the 28th day after transplantation,which indicated that the recipient mice were successfully chimerized.Meanwhile,counts of PLTs on the day 3,7,14,and 21 after transplantation in the experimental group were higher than those in the control group with statistical significances (P<0.05).In addition,hematopoietic function of bone marrow was suppressed in both groups on day 1,3 and 7 after transplantation,but hematopoietic bone marrow hyperplasia was better in the experimental group than in the control group.On day 14 and 21 after transplantation,the hematopoietic function of bone marrow in the two groups was recovered,and the experimental group showed more obvious than the control group.Conclusion:rh TPO can effectively stimulate the production of PLTs and facilitate the recovery of white blood cells and hemoglobin after allo-HSCT,and promote hematopoietic recovery and reconstitution of bone marrow.