一种急性溶血性输血反应小鼠动物模型的建立

A Mouse Model of Acute Hemolytic Transfusion Reactions

目的本研究拟建立一种小鼠急性溶血性输血反应模型,用于探索预防急性溶血性输血反应的发生机制和干预,保障临床输血的安全。方法首先通过交叉配血实验体外验证人和小鼠的血液相合性,然后采用给小鼠静脉输入人O型血浆的方法,建立小鼠急性溶血性输血反应模型,通过外周血液常规检测红细胞计数和形态、血清学检测红细胞致敏情况、病理切片染色和生化分析肾脏和肝脏的损伤水平等几方面来验证急性溶血性输血反应模型的有效性。结果在小鼠接受了不相合血浆的输注后出现血尿,其红细胞计数减少,并且形态发生畸形变,红细胞表面检测出不规则抗体,血浆游离血红蛋白水平升高,释放出游离血红素通过血液循环进入肾脏和肝脏,对肝脏和肾脏造成损伤。结论急性溶血性输血反应小鼠模型成功建立。

Objective To establish a mouse model of acute hemolytic transfusion reactions(AHTRs)to explore the mechanism and intervention of preventing AHTRs and ensure safety of blood transfusion.Methods In vitro blood compatibility of mice and humans were verified by cross matching testing.Then,the mice model of AHTRs was established by intravenously injecting human O-type plasma into mice.The effectiveness of AHTRs model was verified by routine detection of red blood cell count and morphology in peripheral blood,serological detection of red blood cell sensitization,pathological histological section staining and biochemical analysis of kidney and liver injury levels.Results The mice who received mismatched plasma transfusions developed hematuria.Red blood cell count reduced and morphogenesis deformity occurred.Irregular red blood cell antibodies were positive.The levels of plasma free hemoglobin increased.Free hemoglobin exacerbated liver and kidney injury through blood circulation.Conclusion A mouse model of acute hemolytic transfusion reaction was established successfully.