基于PADTM Plus的光活化消毒技术杀灭白色念珠菌的体外实验研究

An in vitro study on killing Candida albicans by photo-activated disinfection technology based on PADTM Plus

目的 研究基于PADTMPlus的光活化消毒技术(photo-activated disinfection,PAD)体外杀灭白色念珠菌的效果。方法 选取白色念珠菌标准株SC5314配制菌悬液。光活化消毒仪输出功率为750 mW,光敏剂为甲苯胺蓝(Toluidine Blue O, TBO)溶液,有效浓度0.75 mg/mL,光源为波长635 nm的LED红光。实验分为空白对照组(P-L-)、单纯光敏剂孵育60 s组(P+T1+L-)、单纯光敏剂孵育120 s组(P+T2+L-)、单纯光照组(P-L+)、PAD孵育60 s组(P+T1+L+)、PAD孵育120 s组(P+T2+L+),光照时间均为60s。根据不同分组的处理方法对白色念珠菌悬液进行处理后接种于ChROMagar培养基,并于37℃环境下培养48 h,菌落计数以每毫升菌落形成单位(CFU/mL)表示。采用统计学方法分析结果。结果 P-L-组、P+T1+L-组、P+T2+L-组与P-L+组的对比其菌落对数的差异均无统计学意义(P>0.05),而P+T1+L+组和P+T2+L+组与P-L-组的对比差异有统计学意义(P<0.01),光敏剂孵育60 s与孵育120 s的效果对比差异无统计学意义(P>0.05)。结论 基于PADTMPlus的PAD技术,在输出功率为750 mW、0.75 mg/mL的TBO溶液孵育时间60 s、635 nm红光照射60 s的情况下,对体外白色念珠菌具有显著杀灭作用,延长孵育时间未见显著提高其灭菌效应。

Objective The study aimed to investigate the in vitro effect of PADTMPlus-based photoactivated disinfection(PAD) technology on killing Candida albicans(C.albicans). Methods C.albicans standard strain(SC5314) was selected for preparation of fungal suspension. The output power was 750 mW, the photosensitizer was Toluidine Blue O(TBO) solution with an effective concentration of 0.75 mg/mL, and the light source was LED red light with a wavelength of 635 nm. The experiment groups divided were as follows: the control group(P-L-), photosensitizer group with an incubation time of the 60 s(P+T1+L-), photosensitizer group with an incubation time of 120s( P+T2+L-), light group( P-L+), PAD group with incubation time of 60 s( P+T1+L+) and PAD group with an incubation time of 120 s( P+T2+L+), all with exposure time of 60 s.C.albicans suspensions were treated according to different treatment methods and were cultured in ChROMagar mediums at 37 ℃ for 48 hours. Colony counts were expressed as colony forming units per milliliter(CFU/mL). Statistical methods were used to analy ze the results. Results The differences among three groups P-L-,P+T1+L-, P+T2+L-, and P-L+ were not statistically significant(P>0.05), while there was statistically significant difference between P+T1+L+group/P+T2+L+group and P-L-group. Both group P+T1+L+ and P+T2+L+ produced statistically significant differences from group P-L-(P<0.01). However, the differences between the effects of incubation with photosensitizer for 60 s and 120 s were not statistically significant(P>0.05). Conclusion PAD technnology mediated by 0.75 mg/mL TBO solution had a significant killing effect on C. albicans at an output power of 750 mW with an incubation time of 60 s and an exposure time of 60 s, and extending the incubation time did not improve its killing effect.