栀子花黄酮纯化工艺及其抗氧化与抗运动疲劳作用研究

Optimization of purification process of flavonoids of Gardenia flower and its anti-oxidant and anti-fatigue effects

采用大孔树脂纯化栀子花黄酮提取物,通过考察不同类型大孔树脂的静态吸附与洗脱性能,确定最佳型号吸附树脂后,采用单因素试验确定最佳纯化工艺条件,另利用体外自由基清除和动物实验考察其抗氧化与抗运动疲劳作用。试验结果显示,最佳纯化条件为上样浓度6.0 mg/mL,上样液pH 5.0,上样流速1.0 mL/min,洗脱液乙醇体积分数70%,洗脱流速2.0 mL/min,洗脱液体积140 mL,产物中黄酮纯度为(76.7±1.2)%。纯化后的栀子花黄酮对羟基自由基和DPPH自由基的半抑制浓度较提取物更低,可明显提高小鼠的乳酸脱氢酶、谷胱甘肽过氧化物酶、超氧化物歧化酶的活力,降低乳酸与丙二醛的浓度水平,并显著延长动物的运动时间,具有较好的抗运动疲劳活力,从而可进一步作为运动食品原料开发。

The flavonoids extract of Gardenia flower was purified by macroporous resin,the optimum type of macroporous resin was determined by comparison of static adsorption-desorption performance in five type macroporous resin. The optimum purification condition was explored by single factor experiment. The effects on antioxidant and anti-fatigue of different products were investigated by radical scavenging in vitro and animal tests. The results showed that the optimum purification conditions were as follows:the loading concentration was 6.0 mg/mL and loading solution pH was 5.0,the loading flow rate was 1.0 mL/min,the volume of elution solution ethanol was 140mL and elution flow rate 2.0 mL/min. The purity of product increased to(76.7±1.2)% with the optimum process.The IC50 of purified Gardenia flower flavonoids on DPPH free radical and hydroxyl radical was lower than the extract.Purified Gardenia flower flavonoids could obviously improve the activities of LDH,GSH-Px and SOD,reduce the concentration level of lactic acid and malondialdehyde,and prolonged exercise time of mice,had better anti-fatigue effect. Therefore,it can be used as raw material for sport food development.