青稞HD-Zip基因家族鉴定及其在非生物胁迫下的表达特性

Identification of HD-Zip Gene Family of Tibetan Hulless Barley and Its Expression Characteristics under Abiotic Stress

同源异型域-亮氨酸拉链(homedomain-leucine zipper,HD-Zip)转录因子广泛参与植物的生长发育和抗胁迫过程。该研究通过生物信息学方法对青稞HD-Zip基因家族进行全基因组分析鉴定,并采用qRT-PCR技术分析非生物胁迫下该基因的表达特性,为深入探讨青稞HD-Zip转录因子的生物学功能及其在高原作物抗逆育种中的应用奠定基础。结果表明:(1)成功从青稞基因组中共鉴定出41个HD-Zip基因家族成员,依次命名为HvvHD-ZipⅠ-1~Ⅳ-13,且这些基因在7条染色体上呈不均匀分布。(2)理化性质分析发现,HvvHD-Zip蛋白包含197~885个不等的氨基酸残基;分子量范围在19914.36~94014.87 Da;亚细胞定位表明HvvHD-Zip蛋白都位于细胞核。(3)根据多序列比对、系统进化、基因结构和保守基序差异将其聚为4个亚家族,各亚家族分类特征与系统聚类结果一致。(4)顺式作用元件预测分析发现,HvvHD-Zip基因启动子中含有11种植物激素和胁迫响应元件。(5)qRT-PCR结果显示,HvvHD-ZipⅠ、Ⅱ、Ⅳ亚家族基因对各胁迫响应明显;与根组织相比,多数HvvHD-Zip基因在叶组织中响应明显(上调或下调);与冷和盐胁迫相比,HvvHD-Zip各基因对旱胁迫响应较强。

Homedomain-leucine zipper(HD-Zip)transcription factors are widely involved in plant growth and development and stress resistance.The genome-wide of HD-Zip gene family of Tibetan hulless barley were analyzed and identified by bioinformatics method,and the expression characteristics of the genes under abiotic stress were analyzed by qRT-PCR technology in this study,which laid a foundation for the in-depth discussion on the biological function of Tibetan hulless barley HD-Zip transcription factor and its application in stress resistance breeding of plateau crop.The results showed that:(1)a total of 41 HD-Zip gene family members were successfully identified in Tibetan hulless barley genome,which were successively named as HvvHD-ZipⅠ-1—Ⅳ-13,and these genes were unevenly distributed on 7 chromosomes.(2)The physical and chemical properties of HvvHD-Zip proteins showed that they contained 197-885 amino acid residues;The molecular weight ranges from 19914.36 to 94014.87 Da.The results of subcellular localization showed that all HvvHD-Zip proteins were located in the nucleus.(3)According to multiple sequence alignment,phylogenetic evolution,gene structure and conservative motif differences,they are grouped into four subfamilies,and the classification characteristics of each subfamily were consistent with the phylogenetic clustering.(4)The prediction analysis of cis-acting elements revealed that the promoter of HvvHD-Zip gene contained 11 cis-acting elements related to plant hormones and stress response.(5)The results of qRT-PCR showed that the HvvHD-ZipⅠ,Ⅱ,Ⅳsubfamily genes responded significantly to each stress.Compared with root tissue,most HvvHD-Zip genes responded significantly in leaf tissue(up-regulated or down-regulated).And compared with cold and salt stress,the HvvHD-Zip genes were more responded to drought stress.