棉花GhGGB基因的克隆与表达分析

Cloning and Expression Analysis of GhGGB Gene in Cotton

蛋白异戊二烯化修饰与植物的抗旱性有关,GGB作为Ⅰ型香叶酰基转移酶的β亚基参与植物的抗逆途径。本研究以‘TM-1’棉花叶片的c DNA为模板,PCR克隆得到GhGGB基因,其开放阅读框为1131 bp,编码377个氨基酸,为亲水性蛋白。此外,通过RT-qPCR技术检测和分析了GhGGB在不同棉花抗旱品种中根、茎、叶中的表达模式,以及GhGGB基因对不同胁迫处理的响应。结果表明GhGGB基因在不同抗旱性的棉花品种中无特异性表达;在相同培养条件下,GhGGB基因受不同胁迫处理后其表达量存在部分差异,其中对干旱处理尤为显著。随干旱胁迫时间的延长,GhGGB基因的表达量逐渐上升。本研究结果为解析棉花的抗逆分子机制提供了基础。

Protein isoprene modify related to drought resistance of plant,GGB asⅠgeranyl acyltransferase beta subunits also participated in the art way in the plant.In this study,using’TM-1’cotton leaf cDNA as template,GhGGB gene was cloned by PCR.Its open reading frame was 1131 bp,encoding 377 amino acids,and it was a hydrophilic protein.In addition,RT-qPCR was used to analyze the expression patterns of GhGGB in roots,stems and leaves of different drought-resistant cotton varieties,as well as the response of GhGGB gene to different stress treatments under the same culture conditions.The results showed that GhGGB gene had no specific expression in cotton varieties with different drought resistance.Under the same culture conditions,the expression level of GhGGB gene was partially different after different stress treatments,especially for drought treatment,and the expression level gradually increased with the extension of drought stress time.The results of this study provide a basis for the molecular mechanism of stress resistance in cotton.