BCRP、LUNX基因mRNA表达水平与非小细胞肺癌患者的病理类型及分期的相关性

Correlation between the mRNA levels of BCRP and LUNX genes and pathological types and stages of patients with non-small cell lung cancer

目的探讨乳腺癌耐药蛋白(BCRP)和肺特异性X蛋白(LUNX)基因的mRNA水平与非小细胞肺癌(NSCLC)患者病理类型及分期的相关性及其对于预后的价值。方法选取2015年6月至2018年6月河南大学淮河医院收治的89例患者为NSCLC组,以同期收治的55例肺良性病变患者为对照,检测两组患者外周血中BCRP、LUNX的mRNA表达水平,并分析其与临床病理特征及预后的相关性。结果NSCLC组外周血中BCRP、LUNX mRNA的阳性表达率显著高于肺良性病变组(P<0.05);NSCLC患者BCRP mRNA的表达与分化程度、TNM分期有关(P<0.05),与性别、年龄、吸烟、病理类型、淋巴结转移无关(P>0.05);其LUNX mRNA的表达与分化程度、TNM分期、淋巴结转移有关(P<0.05),与性别、年龄、吸烟、病理类型无关(P>0.05);BCRP mRNA表达组与LUNX mRNA表达组的总体生存率均显著低于无表达组(P<0.05);分化程度、TNM分期、淋巴结转移、BCRP mRNA表达、LUNX mRNA表达均为影响NSCLC患者预后的因素。结论NSCLC患者外周血中BCRP、LUNX mRNA的水平显著偏高,且BCRP mRNA的表达与分化程度、TNM分期有关,LUNX mRNA的表达与分化程度、TNM分期、淋巴结转移有关,二者可作为评估NSCLC患者预后的独立因素。

Objective To analyze the correlation between the mRNA levels of breast cancer resistance protein(BCRP)and lung-specific X protein(LUNX)genes with pathological types and stages of patients with non-small cell lung cancer(NSCLC)and their significance for prognosis.Methods Eightynine patients with NSCLC admitted to Huaihe Hospital of Henan University between June 2015 and June 2018 were recruited,with 55 patients with benign lung lesions admitted during the same period of time selected as the control group.The mRNA levels of BCRP and LUNX genes were detected in the peripheral blood samples from the two groups,and their correlation with the clinicopathological characteristics and prognosis of the patients was analyzed.Results The expression rates of BCRP and LUNX mRNA in the NSCLC group were significantly higher compared with the control group(P<0.05).The level of BCRP mRNA of the NSCLC patients has correlated with the degree of differentiation and TNM staging(P<0.05),but not with gender,age,smoking,pathological types and lymph node metastasis(P>0.05).The level of LUNX mRNA of them has correlated with the degree of differentiation,TNM staging and lymph node metastasis(P<0.05),but not with gender,age,smoking,and pathological types(P>0.05).Compared with those with no expression,the overall survival rate of patients with BCRP and LUNX expression was significantly lower(P<0.05).The degree of differentiation,TNM staging,lymph node metastasis,and expression of the BCRP and LUNX mRNA may all affect the prognosis of the patients.ConclusionThe levels of BCRP and LUNX mRNA in the peripheral blood of patients with NSCLC are significantly increased.The expression of BCRP mRNA is correlated with the degree of differentiation and TNM staging,whilst the expression of LUNX mRNA is correlated with the differentiation degree,TNM staging and lymph node metastasis.Both may be used as independent predictors for the prognosis of patients with NSCLC.