异养硝化-好氧反硝化菌的分离及脱氮特性

Isolation and Identification of Two Heterotrophic Nitrification-Aerobic Denitrification Strains and Their Nitrogen Removal Characteristics

文章从某垃圾渗滤液高氨氮源区污染的地下水中分离出2株具有异养硝化-好氧反硝化功能的兼性细菌,分别命名为H-8和H-9,经鉴定确定分别为绿针假单胞菌与弗里德里克斯堡假单胞菌。为探究菌株的脱氮特性,作者利用单因素及响应曲面法优化脱氮条件,并在最佳脱氮条件下,将单菌和复合菌应用于实际垃圾渗滤液高氨氮源区污染的地下水中。结果表明,2株HNAD菌的最佳碳源均为丁二酸钠,pH值为7~8、温度范围为22~28℃、碳氮比(C/N)为10~13、转速为100~150 r/min时2株菌脱氮性能最佳,对NH_(4)^(+)-N和TN的去除效率均在96%以上,表明菌株在高氨氮地下水中具有高效的异养硝化好氧反硝化作用;复合菌在72 h的氨氮和总氮去除率达到了81.56%和75.77%,比最高的单菌提高了26.32%和19.4%,证明复合菌比单菌更适用于实际水中的脱氮处理。该菌株高效同步的硝化反硝化性能表明其在处理高氨氮地下水方面有一定的潜力和应用价值。

Two facultative bacteria with heterotrophic nitrification-aerobic denitrification(HN-AD) function were isolated from groundwater polluted by high ammonia nitrogen source of landfill leachate, named H-8 and H-9, respectively. They were identified as Pseudomonas chlororaphis and Pseudomonas frederiksbergensis, respectively. In order to explore the denitrification characteristics of the strain, the single factor and response surface method were used to optimize the denitrification conditions, and under the optimum denitrification conditions, single bacteria and compound bacteria were applied to the groundwater polluted by high ammonia nitrogen source of landfill leachate. The results showed that the best carbon source of the two strains of HN-AD was sodium succinate. When the pH was 7~8, temperature range 22~28 ℃, C/N 10~13 and rotational speed 100~150 r/min, the nitrogen removal performance of the two strains is the best, and the removal efficiency of NH_(4)^(+)-N and TN was above 96%, indicating that the strain has high efficiency of heterotrophic nitrification and aerobic denitrification in high ammonia nitrogen groundwater. The ammonia nitrogen and total nitrogen removal rates of compound bacteria reached81.56% and 75.77% in 72 hours, which were 26.32% and 19.4% higher than those of the highest single bacteria, which proved that compound bacteria were more suitable for denitrification treatment in real water than single bacteria. The high efficiency and simultaneous nitrification and denitrification performance of this strain shows that it has certain potential and application value in the treatment of high ammonia nitrogen groundwater.