聚乙烯醇/γ-聚谷氨酸多孔水凝胶构建研究

Construction of PVA/γ-PGA Porous Hydrogels

本实验采用天然大分子聚乙烯醇(PVA),γ-聚谷氨酸(γ-PGA)和魔芋葡甘聚糖(KGM)为基材,通过反复冻融的物理交联方法构建出半透明、多孔的聚乙烯醇/γ-聚谷氨酸多孔水凝胶。而后通过改变γ-PGA、KGM、PVA浓度以及pH、反复冻融次数、冷冻温度与冷冻时间等单因素实验,并进行响应面法优化,获得了制备γ-聚谷氨酸/聚乙烯醇多孔水凝胶的最佳工艺。实验结果显示,在γ-PGA、KGM与PVA浓度分别在10%、2.5%以及10%,p H为7,冷冻温度-80℃,冷冻时间4小时,反复冻融次数为3次时,所制得的γ-聚谷氨酸/聚乙烯醇多孔水凝胶拥有最佳的溶胀性能,利用扫描电镜和电子万能材料机检测显示所得凝胶拥有丰富的多孔网络结构和良好的机械性能,其溶胀度可达792.6%并且能保水达48小时,可以满足良好敷料的需求。

In this study,natural macromolecular polyvinyl alcohol(PVA),γ-polyglutamic acid(γ-PGA)and konjac glucomannan(KGM)were used as substrates to construct translucent porous hydrogels through physical cross-linking method of repeated freezing and thawing.The preparation was optimized by single-factor experiments(changing the concentration ofγ-PGA,KGM,PVA and pH,the number of repeated freezing and thawing,freezing temperature and freezing time)and the response surface method.The results showed that when the concentrations ofγ-PGA,KGM and PVA are 10%,2.5%and 10%,respectively,pH is 7,freezing temperature is-80℃,freezing time is 4 hours,and the number of repeated freezing and thawing is 3 times,the obtainedγ-polyglutamic acid/polyvinyl alcohol porous hydrogel hold the best swelling properties.Scanning electron microscope and electron universal material machine detection showed that the hydrogels had rich porous network structure and good mechanical properties.The swelling rate reached 792.6%and the hydrogels could retain water for 48 hours,which could meet the needs of ideal dressings.