基于生物信息学分析扩张型心肌病发病机制中潜在的自噬关键基因

Analysis of Potential Autophagy Key Genes in the Pathogenesis of Dilated Cardiomyopathy Based on Bioinformatics

目的通过生物信息学分析扩张型心肌病中潜在的自噬相关基因,为临床治疗提供参考。方法基于GEO数据库中GSE3586数据集筛选扩张型心肌病的mRNA表达谱。通过R软件筛选得到扩张型心肌病自噬相关潜在基因。随后利用蛋白质-蛋白质相互作用(PPI)、相关分析、基因本体论(GO)富集分析和京都基因和基因组百科全书(KEGG)通路富集分析扩张性心肌病自噬相关差异基因。结果在13个扩张型心肌病患者和15个正常人群的室间隔组织样本中鉴定出21个基因与自噬密切相关,其中15个上调基因和6个下调基因。PPI分析显示,扩张型心肌病与自噬相关差异基因之间存在相互作用;GO和KEGG富集分析显示,扩张型心肌病与自噬相关差异基因与细胞自噬的生物学功能和自噬信号通路密切相关。结论自噬相关基因通过调节自噬、凋亡和内分泌抵抗等通路在扩张型心肌病的发病机制中起着重要的作用,为扩张型心肌病后续的治疗和研究提供指导作用。

Objective To analyze the potential autophagy-related genes in dilated cardiomyopathy by bioinformatics,and to provide reference for clinical treatment.Methods The mRNA expression profile of dilated cardiomyopathy was screened based on the GSE3586 data set in the GEO database.Potential genes related to autophagy in dilated cardiomyopathy were screened by R software.Subsequently,protein-protein interaction(PPI),correlation analysis,gene ontology(GO)enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis were used to analyze the differentially expressed genes related to autophagy in dilated cardiomyopathy.Results Twenty-one genes were identified to be closely related to autophagy in ventricular septal tissue samples from 13 dilated cardiomyopathy patients and 15 normal people,including 15 up-regulated genes and 6 down-regulated genes.PPI analysis showed that there was interaction between dilated cardiomyopathy and autophagy-related differential genes.GO and KEGG enrichment analysis showed that the differential genes related to dilated cardiomyopathy and autophagy were closely related to the biological function of autophagy and autophagy signaling pathway.Conclusion Autophagy-related genes play an important role in the pathogenesis of dilated cardiomyopathy by regulating autophagy,apoptosis and endocrine resistance,and provide guidance for subsequent treatment and research of dilated cardiomyopathy.