摘要
This study explored the extraction process of indigo pigment from Baphicacanthus cusia using aqueous enzymatic method for the first time. Through single factor tests and orthogonal tests, this study investigated the effects of extraction time, extraction temperature, enzyme concentration, pH value, and solidliquid ratio on the extraction rate of indigo pigment. The results showed that the extraction rate of indigo reached the highest(0.218%) when the solid-liquid ratio was 1:20, enzyme concentration was 6 g/L, pH value was 5, extraction temperature was 50℃, and extraction time was 3 h. The method had greatly shortened the extraction time, simplified the extraction process, and improved the extraction efficiency while producing little pollution and meeting the green environmental protection requirements.
以南板蓝根为原料,首次探索了水酶法提取靛蓝色素的工艺流程,通过单因素试验和正交试验考察了提取时间、提取温度、加酶量、pH值和料液比等对靛蓝色素提取率的影响。结果表明:在料液比1∶20、加酶量6 g/L、pH值5、提取温度50℃的条件下提取3 h,靛蓝提取率最高,为0.218%。该方法大大缩短了提取时间,简化了提取流程,提高了提取效率,且产生的污染较少,达到了绿色环保要求。
