鸡腿菇菌丝体水提物对四氧嘧啶诱导糖尿病小鼠的影响

Effect of aqueous extract from coprinus comatus mycelium on alloxan induced diabetic mice

目的:探讨鸡腿菇菌丝体水提物(JTGJST)对四氧嘧啶诱导的糖尿病小鼠的降血糖作用及其机制。方法:采用一次性腹腔注射四氧嘧啶(160mg/kg)的方法建立糖尿病小鼠模型。取糖尿病小鼠50只,随机分为模型对照组、盐酸二甲双胍组及鸡腿菇菌丝体水提物(JTGJST)高、中、低剂量组(750 mg/kg、500mg/kg、250 mg/kg)。另取正常小鼠20只,随机分为空白对照组、空白高剂量组。连续灌胃给予样品4周,每周测量小鼠血糖,给予样品结束后测量小鼠血糖、糖耐量、肝糖原、SOD、MDA并对小鼠肝脏、胰腺进行病理检测。结果:与模型对照组相比,鸡腿菇菌丝体水提物各剂量组均可显著降低空腹血糖值(P<0.01),中、高剂量组可增强糖耐量(P<0.01,P<0.05),低、中剂量组可升高肝糖原含量(P<0.05,P<0.01)及SOD活力(P<0.01,P<0.05),各剂量组均可降低肝脏MDA含量(P<0.01),各剂量组均可不同程度的改善肝脏、胰腺受损情况。结论:鸡腿菇菌丝体水提物可有效降低四氧嘧啶诱导的糖尿病小鼠的血糖值,保护受损的肝脏和胰腺,作用机制可能与其抗氧化作用有关。

Objective:To investigate the hypoglycemic effect and mechanism of Coprinus comatus mycelium water extract(JTGJST)on alloxan induced diabetic mice.Methods:The diabetic mice model was established by one time intraperitoneal injection of alloxan(160 mg/kg).Fifty diabetic mice were randomly divided into model control group,metformin hydrochloride group,JTGJST high-dose,mid-dose,low-dos group(750 mg/kg、500 mg/kg、250mg/kg).Twenty normal mice were randomly divided into blank control group and JTGJST blank high-dose group.The mice were gavaged for 4 weeks,and blood glucose was measured every week.Then the blood glucose,glucose tolerance,liver glycogen,SOD and MDA were measured,and the liver and pancreas of mice were pathologically detected.Results:Compared with the model control group,each dose of JTGJST could significantly reduce the fasting blood glucose(P<0.01),JTGJST mid-dose and high-dose group could enhance glucose tolerance(P<0.01,P<0.05),TGJST low-dose and middle-dose group could increase liver glycogen content(P<0.05,P<0.01)and SOD activity(P<0.01,P<0.05),each dose of JTGJST could reduce the content of MDA in liver(P<0.01)and improve the damage of liver and pancreas to varying degrees.Conclusions:JTGJST can effectively reduce the blood glucose level of alloxan induced diabetic mice,protect liver and pancreas.The mechanism may be related to its antioxidant effect.