鞣花酸的抗氧化性及其对PC-12细胞氧化损伤的保护作用研究

Antioxidant activity of ellagic acid and its protective effect on oxidative damage of PC-12 cells

通过鞣花酸清除自由基、抑制亚油酸自氧化、还原铁离子(Fe^(3+))能力实验研究其抗氧化活性;以过氧化氢(H2O2)诱导PC-12细胞建立氧化损伤模型,探索鞣花酸对PC-12细胞氧化应激损伤的保护作用,建立鞣花酸抗氧化活性与对细胞氧化应激损伤之间的联系。结果表明,鞣花酸对羟基自由基(·OH)、超氧阴离子自由基(·O_(2)^(-))、ABTS^(+)·自由基清除的半抑制浓度分别为92.14、36.70、47.91μg/mL;对亚油酸自氧化的抑制率高达90%;对铁离子具有很强的还原能力。鞣花酸对H2O2诱导的PC-12细胞氧化损伤具有良好的保护作用,且与抗氧化活性之间具有良好的相关系。鞣花酸对细胞氧化损伤的保护作用可能与提高细胞的抗氧化能力有关。

The antioxidant activity of ellagic acid was studied by its ability to scavenge free radicals,inhibit autoxidation of linoleic acid and reduce iron ion(Fe^(3+)). The oxidative damage model of PC-12 cells induced by hydrogen peroxide(H2O2)was established to explore the protective effect of ellagic acid on oxidative stress injury of PC-12 cells. The relationship between antioxidant activity of ellagic acid and oxidative stress damage to cells was established. The results showed that the semi inhibitory concentrations of ellagic acid on hydroxyl radical(·OH),superoxide anion radical(·O_(2)^(-)) and ABTS+· radical scavenging were 92.14,36.70 and 47.91 μg/mL respectively.The inhibition rate of linoleic acid autoxidation was up to 90%. And it had strong iron reduction ability. Ellagic acid had a good protective effect on the injury of PC-12 cells induced by H2O2,and it has a good correlation with antioxidant activity. The protective effect of ellagic acid on oxidative damage may be related to improving the antioxidant capacity of cells.