LncRNA-MAFG-AS1在结直肠癌中的表达及临床意义

Expression and clinical significance of LncRNA-MAFG-AS1 in colorectal cancer

目的探讨LncRNA-MAFG-AS1在结直肠癌中的表达及临床意义。方法选取我院行手术切除的结直肠癌组织及对应的癌旁正常组织各80例,应用定量即时聚合酶链锁反应(quantitative real time polymerase chain reaction,qRT-PCR)检测结直肠癌组织及癌旁正常组织中LncRNA-MAFG-AS1的表达水平,并分析其与结直肠癌临床病理特征之间的关系,所有患者随访截止日期到2019年12月31日,应用Kaplan-Meier(K-M)法进行生存分析,采用Cox回归模型分析结直肠癌预后的危险因素。结果结直肠癌组织中LncRNA-MAFG-AS1相对表达量为2.398±0.214,高于癌旁正常组织的1.032±0.132,差异有统计学意义(P<0.05)。当患者TNM分期更高、中高分化及存在淋巴结转移和远期转移时,LncRNA-MAFG-AS1过表达(P<0.05)。K-M生存分析显示高表达患者无进展生存时间(progression-free survival,PFS)为(20.36±3.21)个月,低表达患者为(29.71±4.23)个月;高表达患者的总生存时间(overall survival,OS)为(38.69±6.24)个月,低高表达患者为(54.26±5.14)个月;两组患者的PFS和OS差异有统计学意义(P<0.05)。Cox多因素回归分析显示,TNM分期更高、中高分化及存在淋巴结转移、远期转移以及LncRNA-MAFG-AS1高表达是影响结直肠癌预后的独立危险因素。结论LncRNA-MAFG-AS1在结直肠癌组织中呈高表达,且与结直肠癌患者分化程度、TNM分期、淋巴结和远期转移有关,是影响结直肠癌患者预后的独立风险因素。

Objective To investigate the expression and clinical significance of LncRNA-MAFG-AS1 in colorectal cancer(CRC).Methods A total of 80 cases of CRC tissues that were surgically removed in our hospital and corresponding adjacent normal tissues were selected.The expression levels of LncRNA-MAFG-AS1 in CRC tissues and adjacent normal tissues were detected by qRT-PCR,and their relationship with the clinicopathological features of CRC was analyzed.All patients were followed up until December 31,2019.Kaplan-Meier(K-M)method was used for survival analysis,and Cox regression model was used for risk factor analysis of the prognosis of CRC.Results The relative expression of LncRNA-MAFG-AS1 in CRC tissues was 2.398±0.214,which was higher than that in adjacent normal tissues 1.032±0.132,with a statistical difference(P<0.05).The overexpression of LncRNA-MAFG-AS1 was associated with higher TNM stage,moderate and high differentiation,and the presence of lymph node metastasis and long-term metastasis(P<0.05).K-M survival analysis showed that the progression-free survival(PFS)was(20.36±3.21)months in patients with high expression,and(29.71±4.23)months in patients with low expression;the overall survival(OS)was(38.69±6.24)months in patients with high expression,and(54.26±5.14)months in patients with low and high expression;the difference in PFS and OS between two groups was statistically significant(P<0.05).Cox multivariate regression analysis showed that higher TNM stage,moderate and high differentiation,presence of lymph node metastasis,long-term metastasis and high expression of LncRNA-MAFG-AS1 were independent risk factors for the prognosis of CRC.Conclusion LncRNA-MAFG-AS1 is highly expressed in CRC tissue,and is related to the degree of differentiation,TNM stage,lymph node metastasis and long-term metastasis of CRC patients,which are independent risk factors affecting the prognosis of CRC patients.