摘要
优选熟党参中5-羟甲基糠醛(5-HMF)的最佳提取工艺。采用高效液相色谱法测定熟党参中5-羟甲基糠醛含量为评价指标,通过单因素试验法确定最佳提取方法和最佳提取溶剂,采用L9(34)正交试验设计对5-羟甲基糠醛提取工艺进行优选。提取工艺确定为采用超声波提取法,以甲醇为提取溶剂,液固比100∶1(mL∶g),提取2次,每次0.5 h。优选得到的5-羟甲基糠醛提取工艺简便易行,稳定性好,结果准确可靠。
To optimize the extraction process of 5-hydroxymethylfurfural(5-HMF)in prepared Radix Codonopsis.The content of 5-HMFin prepared Radix Codonopsis was determined by high performance liquid chromatography as the evaluation index.The optimal extraction method and the optimal extraction solvent were determined by single factor test.The L9(3~4)orthogonal test was used to optimize the extraction process of 5-hydroxymethylfurfural.The extraction process was determined as ultrasonic extraction,methanol as extraction solvent,liquid-solid ratio 100∶1(ml∶g),extraction twice,0.5 h each time.The optimized extraction process of 5-HMF in prepared Radix Codonopsis is simple,stable,accurate and reliable.
出处
《科技创新与应用》
2023年第4期67-71,共5页
Technology Innovation and Application
基金
广东省教育厅2021年普通高校重点科研平台项目(2021DZX2082)
2021年广东省科技创新战略专项(“攀登计划”专项)(pdjh2021b0932)
惠州市科技局2020年乡村振兴战略科技创新项目(2020SC0307024)
2021年惠州市农村科技特派员驻镇帮扶项目(2021SCGK00307)。
关键词
熟党参
5-羟甲基糠醛
单因素试验
正交设计试验
提取工艺
prepared Radix Codonopsis
5-Hydroxymethylfurfural
single factor test
orthogonal design test
extraction process
