扬州地区12121例女性HPV感染情况及基因型分布研究

Prevalence and genotype distribution of HPV infection among 12121 females in Yangzhou

目的分析扬州地区不同年龄段女性人乳头瘤病毒(HPV)感染情况及基因型分布,为扬州地区防治HPV感染提供临床依据。方法采用聚合酶链反应体外扩增结合导流杂交技术对12121例女性的宫颈脱落细胞标本进行HPV基因分型检测。分析总体及不同年龄组的HPV感染、HPV单一和多重感染分布情况,以及3种疫苗保护的HPV基因型感染情况。结果扬州地区HPV总感染率为22.71%(2753/12121),以单一高危型感染为主,感染率最高的5种基因型依次为HPV52、16、58、53、51型;HPV感染率在不同年龄组女性间比较,差异有统计学意义(χ^(2)=83.39,P<0.001),<20岁年龄组感染率最高(45.37%);HPV九价疫苗覆盖的基因型感染占比为65.46%。结论扬州地区女性HPV感染以单一高危型为主,以HPV52、16、58、53、51型多见,适龄女性推荐优先选择HPV九价疫苗。

Objective To investigate the human papilloma virus(HPV)infection status,genotype distribution characteristics among females of different age groups in Yangzhou,and to provide a reliable basis for guiding prophylactic HPV vaccination and cervical cancer prevention.Methods Polymerase chain reaction amplification in vitro and flow-through hybridization were used to detect the infection and genotype of HPV in 12121 female cervical epithelial exfoliated cells,HPV infection rates overall and in different age-group were analyzed,as well as the distribution of HPV single and multiple infections,and HPV genotype infection status protected by the three vaccines.Results The overall HPV infection rate in Yangzhou was 22.71%(2753/12121),HPV infection among females in Yangzhou was mainly single high-risk HPV infection.The most common five infection subtypes were HPV52,16,58,53,51.The difference on HPV infection rates among different age groups had statistical significant(χ^(2)=83.39,P<0.001),with the highest peak in the younger than 20-year old group.The infection rate of HPV genotypes covered by nine-valent vaccine accounted for 65.46%of all of the infections females.Conclusion HPV infection among females in Yangzhou is mainly single high-risk HPV infection,and which is mainly caused by HPV52,16,58,53,51 subtypes.Nine-valent vaccine which incorporating more HPV genotypes should be a prior choice for females in Yangzhou.