白花洋紫荆花的化学成分及其胰脂肪酶抑制活性研究

Chemical constituents from the flowers of Bauhinia variegata var.candida and its pancreatic lipase inhibitory activity

为研究白花洋紫荆(Bauhinia variegata var.candida)花的化学成分,以白花洋紫荆花的乙醇提取物为对象,运用葡聚糖凝胶柱层析、MCI柱层析、半制备高效液相色谱等方法进行分离纯化,根据化合物的核磁共振波谱数据进行结构鉴定。结果从白花洋紫荆花中分离纯化得到17个化合物,包括1个葡萄糖苷类、2个苯甲酸类、2个香豆酸类、1个多环酚类,11个黄酮类,分别鉴定为β-D-甲基葡萄糖苷(1)、对羟基苯甲酸甲酯(2)、对甲氧基苯甲酸(3)、对香豆酸(4)、对甲氧基桂皮酸(5)、pacharin(6)、柚皮素(7)、异牡荆苷(8)、山柰酚(9)、6-羟基山奈酚3-O-葡萄糖甙(10)、山柰酚-3-O-鼠李糖苷(11)、百蕊草素(12)、kaempferol 3-O-α-L-rhamnopyranosyl-(1→6)-β-D-lucopyranoside(13)、槲皮素(14)、3-O-甲基槲皮素(15)、quercetin-3-O-α-L-rhamnopyranosyl-(1→6)-β-D-glucopyranoside(16)、quercetin-3-O-(6″-O-α-L-rhamnopyranosyl)-β-D-glucoside(17)。本研究得到的化合物均为首次从该植物中分离得到。共筛选了化合物10、12、13、14、17的胰脂肪酶活性,结果显示该5个化合物对胰脂肪酶均具有一定的抑制活性。

To study the chemical constituents from the flowers of B.variegata var.candida.The constituents were isolated from the ethanol extract of flowers of B.variegata var.candida and purified by column chromatography Sephadex LH-20,MCI and semi-preparative high performance liquid chromatography.The structures of the compounds were identified by NMR spectroscopic data.A total of 17 compounds were isolated from the flowers of B.variegata var.candida,including one glucoside,two benzoic acids,two coumarins,one polycyclic phenols,eleven flavonoids,and the structures were identified as methylβ-D-glucopyranoside(1),methyl-4-hydroxybenzoate(2),4-methoxybenzoic acid(3),p-coumaric acid(4),p-methoxy cinnamic acid(5),pacharin(6),naringenin(7),isovitexin(8),kaempferol(9),6-hydroxykaempfero-3-O-glucoside(10),kaempferol-3-O-α-L-rhamnoside(11),kaempferol 3-O-neohesperidoside(12),kaempferol 3-O-α-L-rhamnopyranosyl-(1-2)-β-D-lucopyranoside(13),quercetin(14),3-O-methylquercetin(15),quercetin-3-O-α-L-rhamnopyranosyl-(1→6)-β-D-glucopyranoside(16),quercetin-3-O-(6″-O-α-L-rhamnopyranosyl)-β-D-glucoside(17).All the compounds were obtained from the plant for the first time.Compounds 10,12,13,14,17 were screened for pancreatic lipase inhibitory activity,and the results showed that the five compounds have certain inhibitory activity on pancrelipase.