复方阿莫西林乳房注入剂无菌检查方法的优化

Optimization of Sterility Test Method of Compound Amoxicillin Intramammary Infusion

将阿莫西林乳房注入剂无菌检查方法由直接接种法优化为薄膜过滤法。采用肉豆蔻酸异丙酯将样品破乳,与pH 6.0磷酸盐缓冲液混合进行萃取,取萃取后的水相薄膜过滤,采用pH 7.0无菌氯化钠-蛋白胨缓冲液进行冲洗,冲洗完毕加入硫乙醇酸盐液体培养基和胰酪大豆胨液体培养基,定量加入青霉素酶破坏残余的阿莫西林,按照规定条件培养。该方法与直接接种法相比,操作简单,可靠性增强,结果判断准确性提高。

The sterility test method of amoxicillin intramammary infusion was optimized from direct inoculation method to membrane filtration method. The sample was demulsified with isopropyl myristate, mixed with pH 6.0 phosphate buffer solution for extraction. The aqueous phase was filtered through membrane after extraction. The membrane was washed with pH 7.0 sterile sodium chloride-peptone buffer solution, added with thioglycollate liquid medium and tryptophan soybean peptone liquid medium after washing, added penicillin enzyme quantitatively to destroy residual amoxicillin, and cultured according to the specified conditions. Compared with the direct inoculation method, the optimized method is simple, reliable and accurate.