高效液相色谱法检测育肥猪配合饲料中孟布酮

Detection of Menbutone in Compound Feed for Finishing Pigs by HPLC

应用高效液相色谱技术,建立了育肥猪配合饲料中孟布酮的检测方法。样品经乙腈提取后,通过WAX固相萃取柱净化;采用0.5%磷酸乙腈溶液作为流动相进行梯度洗脱,经C18色谱柱分离,通过二极管阵列检测器进行测定。在0.2~10μg/mL的范围内线性关系良好,相关系数大于0.999。在0.2,0.4,2 mg/kg添加水平下,平均回收率为78.1%~90.6%,相对标准偏差均小于10%。检测限为0.06 mg/kg,定量限为0.2 mg/kg,该法准确、简单、快速。

A HPLC method was developed to determine menbutone in compound feed for finishing pigs. The samples were extracted by acetonitrile and purified by a WAX solid phase extraction column. The HPLC separation was performed on C18column with a linear gradient elution program of 0.5% phosphoric acid acetonitrile solution as the mobile phase. PDA was used for the selective detection. There was a good line correlation in the range of 0.2~10 μg/mL, the correlation coefficient was greater than 0.999. The average recorvery was 78.1%~90.6% and RSD were both less than 10%. The limit of detection was 0.06 mg/kg and limit of quantification was 0.2 mg/kg. The method was accurate, convenient and fast.