鸡滑液支原体的分离鉴定与多位点序列分型

Isolation,Identification and Multilocus Sequence Typing of Mycoplasma synoviae

为研究国内鸡滑液支原体(Mycoplasma synoviae,MS)的流行情况,2016-2021年间从山东、四川、河北等9省份共计分离到54株MS,通过多位点序列分型(multilocus sequence typing,MLST)方法对分离株进行基因分型,同时结合国内外的分离株信息,进一步分析了不同菌株间的进化关系。结果表明,54株MS分离株可分为9种ST型,分别是34、44、93、95、96、102、103、148、153,主要流行的ST型为ST34和ST93;按照进化关系可分为A-I共9类,其中最主要的流行株为A类和E类。通过对国内外MS流行株进行遗传进化分析,可依据地域将其分为亚洲株、欧洲株、北美株和其他株,其中中国分离株有独特的地域进化分支。本研究结果丰富了国内的MS流行病学数据,对防控与净化MS具有重要意义。

This study aims to research the epidemic situation of Mycoplasma synoviae(MS)in China.Between 2016 and 2021,54 MS strains were isolated from nine provinces,including Shandong,Sichuan,and Hebei.These strains were genotyped using multilocus sequence typing(MLST).The evolutionary relationships of different strains were analyzed using gene sequences with china isolates and foreign isolates.The results indicated that 54 MS strains were classified into nine ST types,including 34,44,93,95,96,102,103,148,and 153.According to the evolutionary relationship,they could be divided into nine groups from A to I,with the most popular strains belonging to groups A and E.The phylogenetic analysis of MS epidemic strains worldwide revealed that they might be classified as Asian strains,European strains,North American strains,and other strains.Among them,Chinese strains have a unique evolutionary relationship.The results of this study enriched the epidemiological data and were of great significance for the prevention and eradication of MS in China.