基于RIL群体的玉米穗行数相关QTLs定位与分析

Mapping and Analysis of QTLs Related to Ear Row Number in Maize Based on RIL Population

定位不同环境下影响玉米穗行数的稳定表达的QTLs是玉米分子标记辅助选择的基础和前提。本研究以X 178和农系531为亲本构建的283份重组自交系(RIL)为实验材料,利用SSR分子标记技术和复合区间作图法(ICIM-ADD)分别对辛集、保定的玉米穗行数进行QTL的定位与分析。结果表明,共检测到19个与玉米穗行数有关的QTLs,分布在1、2、4、6、7、9号染色体上。单个QTL表型贡献率为3.4%~9.1%;辛集检测到8个,单个QTL表型贡献率为3.9%~11.77%。其中有两个位点分别在保定和辛集同时被检测到:qKRE 1-B-1和qKRE 3-X-9,其平均贡献率分别为5.21%和9.16%。保定检测到11个,这些在不同环境下检测到的QTLs为玉米穗行数的QTL精细定位提供了理论依据。

Locating QTLs that affect stable expression of ear row number in maize under different environments is the basis and prerequisite of molecular marker-assisted selection in maize.In this study,283 recombinant inbred lines(RIL)from X 178 and NongXi 531 were used as experimental materials.QTL mapping and analysis of maize ear row number in Xinji and Baoding were carried out by SSR molecular marker and ICIM-ADD.The results showed that a total of 19 QTLs related to ear row number of maize were detected and distributed on chromosome 1,2,4,6,7 and 9.A single QTL phenotype contributed 3.4%-9.1%.Eight QTLs were detected in Xinji,and the phenotypic contribution of a single QTL was 3.9%-11.77%.Two loci,qKRE 1-B-1 and qKRE 3-X-9,were detected in Baoding and Xinji,with an average contribution rate of 5.21%and 9.16%,respectively.Eleven QTLs were detected in Baoding,and these QTLs detected in different environments provided theoretical basis for fine mapping of maize ear rows.