StEFR1正调控马铃薯对晚疫病的抗性

StEFR1 regulates late blight resistance positively in potato(Solanum tuberosum)

马铃薯晚疫病是毁灭性卵菌病害,对我国乃至全球的农业生产造成巨大的经济损失。本试验结合生物信息学分析、表达模式和功能验证,分析了LRR-RLK家族成员StEFR1调控晚疫病抗性的作用和潜在的调控机制。进化分析表明,StEFR1与拟南芥中功能已知的AtEFR序列相似度为53.9%。接种晚疫病菌3 d和elf18处理3 h,‘大西洋’离体叶片中StEFR1的表达分别上调至对照的1.87倍和2.31倍。瞬时过表达StEFR1的叶片受到晚疫病菌侵染时抗性增强,表现在叶片病斑面积较对照减小,而叶片细胞活性较对照增强。此外,与野生型相比,过表达StEFR1的叶片中3个PTI标记基因、SA和JA信号通路相关基因差异表达,且呈不同程度的显著上调,而ET信号通路相关基因的表达量无明显变化。综上所述,StEFR1参与晚疫病菌诱导的PTI抗性,且调控SA和JA激素信号相关基因的表达,对晚疫病起正调控作用。本文为深入研究StEFR1调控晚疫病免疫反应的分子机制奠定了基础,并为晚疫病分子育种研究提供重要参考。

Potato late blight is a devastating oomycete disease,which causes great economic losses to agricultural production.In this study,we analyzed the role and potential regulatory mechanism of StEFR1 in regulating late blight resistance by the relative expression pattern and functional verification combined with the bioinformatics methods.Evolutionary analysis showed that the sequence similarity between StEFR1 and AtEFR was 53.9%.After inoculated with Phytophthora infestans for 3 days and treatment with elf18 for 3 hours,the relative expression level of StEFR1 in isolated leaves of Atlantic was upregulated to 1.87 times and 2.31 times compared with the control,respectively.The late blight resistance significantly increased after the overexpression of StEFR1 in the isolated leaves of Atlantic by agrobacterium infiltration method.Compared with the control,the area of leaf lesion size decreased and the activity of leaf cells increased.And the marker genes of PTI,SA,and JA signaling pathways in overexpressed leaves were significantly up-regulated to varied degrees,while the relative expression levels of ET related-genes did not change significantly.In conclusion,StEFR1 was involved in the PTI resistance and regulated the relative expression levels of SA and JA hormone signaling related genes,suggesting that St EFR1 positively regulated the potato late blight.This study lays a foundation for further revealing the molecular mechanism of StEFR1 in regulating the immune response and provides important reference for the molecular breeding of late blight.