摘要
为研究南极磷虾油的抗炎作用,以脂多糖(LPS)诱导的RAW264.7细胞为炎症模型,通过细胞形态、细胞吞噬能力、髓过氧化物酶(MPO)活力、促炎细胞因子(TNF-α、IL-1β和IL-6)等指标,评价南极磷虾油的抗炎能力,并通过炎症因子及炎症关键酶(iNOS和COX-2)基因的表达,研究其抗炎机理。结果表明,南极磷虾油可以显著缓解LPS引起的细胞分化,抑制细胞吞噬能力和MPO活力的增强,以及炎症因子分泌量的升高(尤其是TNF-α)。实时荧光定量PCR(qRT-PCR)的检测结果表明,南极磷虾油可以显著平息LPS引发的炎症关键酶和炎症因子基因的活化。结论:南极磷虾油具备抗炎活性,该活性与其对i NOS和COX-2的调控作用相关。
In order to study the anti-inflammatory effect of Antarctic krill oil,the LPS-induced RAW264.7 cells were taken as the inflammatory model,the anti-inflammatory ability of Antarctic krill oil was evaluated by cell morphology,cell phagocytosis,myeloperoxidase(MPO)activity and pro-inflammatory cytokines(TNF-α,IL-1βand IL-6),and its anti-inflammatory mechanism was preliminarily studied by the genes expression of pro-inflammatory cytokines and key inflammatory enzymes(i NOS and COX-2).The results showed that Antarctic krill oil could significantly alleviate the cell differentiation induced by LPS,inhibit the increase of cell phagocytosis,MPO activity and the secretion of inflammatory factors(especially TNF-α).The q RT-PCR detection indicated that Antarctic krill oil could significantly quell the activation of key inflammatory enzymes and inflammatory factor genes induced by LPS.This study shows that Antarctic krill oil has anti-inflammatory activity,which is related to its regulation of iNOS and COX-2.
作者
郑振霄
朱凯
戴志远
Zheng Zhenxiao;Zhu Kai;Dai Zhiyuan(Institute of Sea Food,Zhejiang Gongshang University,Hangzhou 310012;State Key Laboratory of Aquatic Products Processing of Zhejiang Province,Zhejiang Gongshang University,Hangzhou 310012;Collaborative Innovation Center of Seafood Deep Processing,Zhejiang Gongshang University,Hangzhou 310012)
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2023年第1期23-31,共9页
Journal of Chinese Institute Of Food Science and Technology
基金
浙江省自然科学基金项目(LQ22C200008)。
