基于氨基酸代谢的复合茶降脂作用研究

Studies on the Lipid-lowering Effect of Compound Tea Based on Amino Acid Metabolism

为了明确复合茶的降脂作用,建立高脂饮食小鼠模型,采用UPLC-MS/MS检测、多元统计分析以及自建数据库相结合的方法研究复合茶样品间代谢组的差异度。设置正常模型组(CK)、高脂饮食组(NK)和复合茶干预组(DH),以血脂康胶囊作为阳性对照组(YK),对比复合茶对高脂血症小鼠肝脏中的氨基酸代谢的影响。结果表明:经复合茶干预后,高脂饮食诱导小鼠肝脏中的L-高胱氨酸、N-乙酰基蛋氨酸、D-丙氨酰-D-丙氨酸、S-腺苷蛋氨酸、L-天冬酰胺和L-酪氨酸甲酯等13种氨基酸代谢物呈下调趋势,且显著富集在半胱氨酸和甲硫氨酸代谢途径中。对比阳性药物组,血脂康干预后仅N-乙酰天冬氨酸、N-异戊酰氨基乙酸和丙甘氨酸等5种氨基酸代谢物显著下调。说明复合茶对高脂血症小鼠的肝脏氨基酸代谢物及通路具有一定的调节作用,且效果优于阳性药物血脂康。研究结果为利用代谢组学技术,探究复合茶中氨基酸及其衍生物的降脂机理提供参考。

In order to study the lipid-lowering effect of compound tea,the high-fat mice model was established by high-fat diet.Based on the method of UPLC-MS/MS detection platform,multivariate statistical analysis and self-built database,the difference degree of metabolism group among samples was studied.In this experiment,the normal model group(CK),high-fat diet group(NK)and compound tea group(DH)were set up,and the Xuezhikang capsule was used as the positive drug group(YK)to compare the effects of compound tea on the metabolism of amino acids in the liver of hyperlipidemic mice.The results showed that a total of 13 amino acid metabolites such as L-homocystine,N-acetylmethionine,D-alanyl-D-alanine,S-adenosylmethionine,L-asparagine and L-tyrosine methyl ester in the liver of mice induced by high-fat diet showed a downward trend,which were significantly enriched in the cysteine and methionine metabolism after the intervention of compound tea.Compared with the YK group,only five of amino acid metabolites such as N-acetylaspartate,N-isovaleric acid and alanine were significantly down-regulated after treatment of Xuezhikang.These fundings indicated that compound tea had a certain regulatory effect on liver amino acid metabolites and pathways in hyperlipidemia mice,which effect was better than the positive drug Xuezhikang.The purpose of this study is to explore the mechanism of reducing lipid of amino acids and their derivatives in compound tea by using metabonomics technology,and to provide some reference for the development and utilization of compound tea.