红树莓和黄树莓果实中的代谢物比较分析

Comparative Analysis of Metabolites in Red Raspberry and Yellow Raspberry Fruits

为探究黄树莓和红树莓成熟果实中的代谢物差异,对其进行非靶向LC-MS代谢组学分析。在正离子模式下,共检测到708种(98种已知)差异代谢物。在负离子模式下,共有780个(45种已知)差异代谢物。通过KEGG数据库的代谢富集分析,红树莓和黄树莓果实中共有1566个差异代谢物质富集在新陈代谢、遗传信息处理、环境信息处理和人类疾病这4大类别上。这些差异代谢物质共富集在66个通路上,代谢途径富集的差异代谢物最多(34种)。此外,黄树莓果实中的飞燕草素葡萄糖苷、脱落酸、氢化肉桂酸和橙皮素较红树莓果实中分别下调了-1.496,-1.632,-1.527,-0.477倍。而原叶绿酸在黄树莓果实中上调了10倍以上。研究结果为不同树莓果实中特定化合物的开发和利用提供理论依据。

To explore the differences of metabolites in the mature fruits of yellow raspberry and red raspberry,nontargeted LC-MS metabolomics analysis was carried out.A total of 708(98 known)significantly differentially expressed metabolites were detected in the positive ion mode.In the negative ion mode,there were 780(45 known)significantly differentially expressed metabolites.Through the metabolic enrichment analysis of KEGG database,1566 differentially expressed metabolites in red raspberry and yellow raspberry fruits are enriched in four categories:metabolism,genetic information processing,environmental information processing and human diseases.These differentially expressed metabolites were enriched in 66 pathways,and the metabolic pathways(ko01100)enriched the most differential metabolites(34 species).In addition,delphinidin 3-glucoside,(s)-abscisic acid,hydrocinnamic acid and hesperetin in yellow raspberry fruits were-1.496,-1.632,-1.527 and-0.477-fold lower than those in red raspberry fruits,respectively.Protochlorophyllide was up-regulated more than 10 times in yellow raspberry fruit.The results provide theoretical basis for the development and utilization of specific compounds in different raspberry fruits.