ICP-MS法测定中药龙骨中7种元素的含量

Determination of 7 elements in Keel by ICP-MS

采用氢氟酸-高氯酸-硝酸的三酸体系湿法消解处理龙骨样品,以Rh作为内标进行基体效应补偿,通过电感耦合等离子体质谱法(ICP-MS)首次测定龙骨中Ni、Cr、Sr、Cu、Zn、Pb、Co等7种元素的含量。方法学验证结果显示该法测定结果可靠,Ni、Cr、Sr、Cu、Zn、Pb、Co各元素质量分数分别为11.71μg/g、18.03μg/g、741.38μg/g、8.11μg/g、39.67μg/g、7.67μg/g、2.26μg/g。龙骨中含有丰富的Sr、Zn等人体必需的微量元素,Pb含量未超标。检测结果为龙骨药效物质的基础元素研究、元素含量质量控制,及进一步开发利用其药效提供一定的科学依据。

The Keel samples were dissolved with microwave digesting,wet diges-ting and dry ashing,respectively.The matrix effect was corrected by the in-line addition of internalstandard(Rh).ICP-MS was applied to simultaneously determine the elements of Ni、Cr、Sr、Cu、Zn、Pb and Co in Keel.The contents of Ni、Cr、Sr、Cu、Zn、Pb、Co were 11.71μg/g、18.03μg/g、741.38μg/g、8.11μg/g、39.67μg/g、7.67μg/g、2.26μg/g.Our results indicate that Keel contains abundant human essential trace elements such as Sr、Zn,and the contents of Pb heavy metals do not exceed the criterion.This method was proved to be high precision,repeatability and stability,and accurate,it provides scientific basis for the quality control of keel and the study of pharmacodynamic material basis.