半夏泻心汤通过抑制炎症抑制小鼠溃疡性结肠炎

Banxia Xiexin Decoction inhibited ulcerative colitis in mice by inhibiting inflammation

[目的]观察半夏泻心汤古方剂量及现代临床常用剂量对采用葡聚糖硫酸钠(DSS)自由饮用诱导建立的溃疡性结肠炎(UC)模型小鼠(DSS-UC小鼠)炎症因子的影响,探索其合理的临床用量并从免疫角度探讨其治疗溃疡性结肠炎的作用机制。[方法]将32只C57BL6/J小鼠随机分为空白组8只、模型组8只、半夏泻心汤古方组8只和半夏泻心汤药典组8只。采用2.5%的DSS自由饮用7 d诱导溃疡性结肠炎模型,造模同时对各组小鼠每日对应给予生理盐水、半夏泻心汤古方量方、半夏泻心汤药典量方灌胃14 d。随后苏木精-伊红(HE)染色观察结肠组织病理学变化,酶联免疫吸附测定法(ELISA)检测血清及结肠组织中炎症因子水平。[结果]半夏泻心汤可显著恢复DSS-UC模型小鼠的体质量、降低疾病指数(DAI)。升高DSS-UC小鼠血清及结肠组织中5-羟色胺(5-HT)、白介素(IL)-10水平,降低血清及结肠组织中IL-4、肿瘤坏死因子(TNF)-α水平,但对IFN-γ水平无显著改善。[结论]半夏泻心汤可以通过升高DSS-UC小鼠血清及结肠组织中IL-10、5-HT水平,降低血清及结肠组织中IL-4、TNF-α水平进而改善DSSUC小鼠肠道炎症反应,改善组织损伤情况,从而发挥治疗UC的治疗作用,且以古方量效果为佳。

[Objective]To observe the effects of Banxia Xiexin Decoction dose and modern clinical dose on inflammatory factors in mice with ulcerative colitis induced by dextran sulfate sodium(DSS-UC)free drinking.To explore the reasonable clinical dosage and immune mechanism of ulcerative colitis.[Methods]The 32 C57BL6/J mice were randomly divided into control group(n=8),model group(n=8),Banxia Xiexin Decoction ancient prescription group(n=8)and Banxia Xiexin Decoction pharmacopoeia prescription group(n=8).Ulcerative colitis model was induced by 2.5%DSS free drinking for 7 days.At the same time,the mice in each group were given normal saline,normal saline,Banxia Xiexin Decoction Gufang and Banxia Xiexin Decoction pharmacopoeia by using gastric gavage for 14 days.Histopathology was observed with hematoxylin-eosin staining(HE)and enzyme linked immunosorbent assay(ELISA)was then used to detect the levels of inflammatory factors in serum and colon tissues.[Results]Banxia Xiexin Decoction could significantly restore body weight and decrease disease activity index(DAI)index of DSS-UC model mice.The levels of 5-hydroxytryptamine(5-HT)and interleukin-10(IL-10)in serum and colon tissue of DSS-UC model mice were increased,while interleukin-4(IL-4)and tumor necrosis factor(TNF-α)were decreased,but interferon-gamma(IFN-γ)level was not significantly effect.[Conclusion]Banxia Xiexin Decoction could improve intestinal inflammatory response and tissue damage of DSS-UC mice by increasing the levels of IL-10 and 5-HT in serum and colon tissue,and reducing the levels of IL-4 and TNF-αin serum and colon tissue,which plays a therapeutic role in treating UC.Furthermore,the effect of Gufang dose is better.