两种猪伪狂犬gE抗体ELISA检测试剂盒效果评价

Effect Evaluation of Two ELISA Kits for Detecting gE Antibody in Swine Pseudorabies

目的:为了评估A、B两种猪伪狂犬gE抗体ELISA检测试剂盒的检测效果,为猪伪狂犬gE抗体ELISA检测试剂盒提供选择依据。方法:试验将12份待检血清样品进行检测并计算A、B试剂盒的符合率和阳性率;通过对3份留样血清样品进行梯度稀释、设置平行实验进行检测,评估两种试剂盒的重复性、灵敏度和稳定性。结果:用两种试剂盒检测,样品的阳性率均为33.3%,符合率为100%;通过稀释留样血清样品的检测和平行试验发现:用A试剂盒检测两份样品,检出显示阳性的最低稀释度分别为1∶64和1∶16;用B试剂盒检测2份样品,检出显阳性的最低稀释度分别为1∶32和1∶8。结论:两种试剂盒重复性好,均适用于临床样品的PRV gE抗体检测,但A试剂盒灵敏度高于B试剂盒,而B试剂盒稳定性更高且价格更低。说明生产实践中应对A、B试剂盒的重复性、灵敏度、稳定性和价格因素进行综合考量,合理选择性价比更高试剂盒。

Objective: To evaluate the detection effect of ELISA kit for gE antibody of swine pseudorabies A and B, and to provide a basis for selection of ELISA kit for gE antibody of swine pseudorabies. Methods: 12 serum samples were tested and the coincidence rate and positive rate of kit A and B were calculated;the repeatability, sensitivity and stability of the two kits were evaluated by gradient dilution of 3 retention serum samples and parallel test. Results: The positive rate of samples was 33.3% and the coincidence rate was 100% when tested with two kinds of kits;through the detection of diluted serum samples and parallel test, it was found that the lowest dilution of positive results were 1∶64 and 1∶16 respectively when two samples were detected with kit A;two samples were tested with kit B, and the minimum dilution of positive reaction was 1∶32 and 1∶8, respectively. Conclusion: The two kits have good repeatability and are suitable for the detection of PRV gE antibody in clinical samples. However, the sensitivity of kit A is higher than that of kit B, while kit B has higher stability and lower price. It indicates that the repeatability, sensitivity, stability and price factors of A and B reagent kits should be comprehensively considered in production practice, and a more cost-effective kit should be reasonably selected.