激光共聚焦扫描显微镜载样台改造及使用

Modification and use of sample holder for laser confocal scanning microscope

激光共聚焦扫描显微镜已经成为了生物科学研究中不可或缺的大型高端光学成像仪器。但由于生物样品来源和类型丰富,而购置的激光共聚焦显微镜单一形式载样台不能满足各类生物学样品的使用。本工作在Leica TCS SP8激光共聚焦显微镜载样台的基础上,设计了一种能够避免原装载样台造成物镜撞击载物台并且能够更好的适应生物科研中各类成像样本的新载样台。改造结果显示更换载样台后,极大的降低了载物台撞显微镜物镜的概率,避免了显微镜物镜的损伤和因载样台的限制而不能进行成像造成样本的浪费,并且更加方便显微镜的日常使用。该次载样台改造的尝试为国内其他科研平台或仪器制造商提供了设计一种更好适应不同样品和物镜友好样品台的思路,具有很大的实用价值和借鉴意义。

Confocal laser scanning microscope(CLSM)is an indispensable large optical imaging tool in biological science research.Due to the variety of sample types and sources in biological scientific research,singleform sample holder of LCSM cannot meet the requirements of various samples in biological research.This work is based on the sample holder of Leica TCS SP8 LCSM,which solves the problem of the objective lens hitting the sample holder,and can better adapt to various imaging samples in biological research.The results show that after replacing the sample holder,the damage of the sample holder bumping into the objective is greatly reduced,the waste caused by cannot imaging of some precious samples due to the limitation of sample holder.This modification is of great practical value and referential significance for scientific research platforms or instrument manufacturers.