153份新疆冬小麦品种3个条锈病抗性基因的分子检测

Molecular Detection of Three Yr Genes in 153 Winter Wheat Varieties from Xinjiang

【目的】研究条锈病抗性基因Yr 9、Yr 26、Yr Tp1在新疆153份冬小麦品种中的分布,为新疆小麦抗病育种提供理论依据。【方法】利用SSR分子标记,采用PCR扩增和聚丙烯酰胺凝胶电泳方法,检测供试材料中是否含有小麦条锈病抗性基因Yr 9、Yr 26和Yr Tp1。【结果】在123份冬小麦地方品种和30份冬小麦育成品种中,Yr 9基因的检出率分别为78.86%和63.33%,Yr 26基因的检出率分别为95.12%和73.33%,Yr Tp1基因的检出率分别为88.62%和60%。在123份冬小麦地方品种中,有87份供试材料能同时检测出3个条锈病抗病基因,占比70.73%;28份供试材料能同时检测出2个条锈病抗性基因(Yr 9+Yr 26、Yr 9+Yr Tp1、Yr 26+Yr Tp1),占比22.76%;1份供试材料只能检测出Yr 9基因;5份供试材料只能检测出Yr 26基因。在30份冬小麦育成品种中,15份供试材料可以同时检测出3个条锈病抗性基因,占比50%;7份供试材料可以同时检测到2个抗性基因(Yr 9+Yr 26、Yr 26+Yr Tp1)的材料,占比23.33%。【结论】携带Yr 9、Yr 26、Yr Tp1小麦条锈病抗性基因的新疆冬小麦种质资源丰富,可以作为抗病育种的中间材料。

【Objective】To clarify the distribution of stripe rust resistant genes Yr 9,Yr 26 and Yr Tp1 in 153 winter wheat varieties from Xinjiang with a view to providing theoretical basis for resistance breeding of Xinjiang winter wheat.【Methods】The SSR marker of stripe rust resistant genes,polymerase chain reaction and polyacrylamide Gel electrophoresis were used to detect whether the tested wheat varieties contain Yr 9,Yr 26 and Yr Tp1 genes.【Results】The percentage of 123 winter wheat landraces and 30 cultivars from Xinjiang that carried Yr 9 was 78.86%and 63.33%,respectively;Yr 26 was 95.12%and 73.33%,respectively;Yr Tp1 was 88.62%and 60%,respectively;In 123 winter wheat landraces,we identified that all the three resistant genes in 87 varieties(70.73%),two resistant genes—the gene recombination types including Yr 9+Yr 26,Yr 9+Yr Tp1,Yr 26+Yr Tp1-in 28 varieties(22.76%),only Yr 9 in 1 variety,only Yr 26 in 5 varieties;In 30 winter wheat cultivars,we detected all the three resistant genes in 15 varieties(50%),two resistant genes—the gene recombination types including Yr 9+Yr 26andYr 26+Yr Tp1 in 7 varieties(23.33%).【Conclusion】Above all,it is concluded that the winter wheat germplasm in Xinjiang carrying the Yr genes are abundant and these germplasms can be used as intermediate materials in disease resistant breeding.