MBBR填料快速挂膜的菌群特征与校园景观池水净化试验

Bacterial diversity in rapid biofilm culturing of MBBR fillers and campus landscape pond water purification

采用接种排泥和间歇性曝气联合进行MBBR挂膜,并探究不同尺寸、不同填充率对填料生物挂膜、微生物群落结构和污染物去除效率的影响。采用联合法挂膜培养,兼顾两者优点,可以在15 d实现挂膜,附着的生物膜对有机负荷和污染波动具有较好耐受性,对污染物的去除效果较好。16S rRNA基因高通量测序结果显示,不同尺寸填料上生物膜中的优势菌均为Candidatus Saccharibacteria,占比均超过40%。以分别投放有两种尺寸的挂膜填料的MBBR处理校园景观池水,填充率为30%的小填料具有更高的污染物去除能力,8 h后出水水质可达到地表水Ⅳ类水质标准。

The combination of inoculation sludge discharge and intermittent aeration was used for MBBR biofilm culturing,and the effects of different sizes and filling rates on biofilm culturing,microbial community and pollutants removal efficiency were investigated.The biofilm could form in 15 d with the combined method,which showed the advantages of the two methods.The attached biofilm had a good tolerance to organic load and pollution fluctuation with a high pollutant removal efficiency.The 16S rRNA gene MiSeq result indicated that the dominant bacteria in biofilms with different sizes of fillers were Candidatus Saccharibacteria,accounting for more than 40%.The campus landscape pool water was treated by MBBR with two sizes fillers with biofilm,respectively.The smaller fillers with a filling rate of 30%had a higher pollutant removal efficiency,and the effluent could meet the Class IV Water Standard of surface water within an 8 h treatment.