鬼点灯药材脂溶性成分及抗氧化活性研究

Liposoluble Constituents and Antioxidant Activity of Bothriospermum Tenellum

目的研究鬼点灯药材脂溶性成分及其抗氧化活性。方法采用气相色谱-质谱联用法检测鬼点灯药材的脂溶性成分(石油醚提取物),并参考标准质谱谱库数据进行鉴定;采用DPPH和ABTS+自由基清除实验检测其抗氧化活性,均以维生素C作阳性对照。结果共检测出46个化学成分,鉴定出22个主要化学成分,含量较高的包括棕榈酸(26.19%)、反式-13-十八碳烯酸(22.92%)、生育酚(10.03%)、5,7-二甲氧基香豆素(8.80%)、肌醇(4.55%)等。药材脂溶性成分对DPPH自由基的半数抑制浓度(IC50)为208.69μg/mL,对ABTS^(+)自由基的IC_(50)为146.00μg/mL,阳性对照的IC_(50)分别为25.80μg/mL及21.20μg/mL。结论首次鉴定出鬼点灯脂溶性成分主要含有脂肪酸类、脂肪醇类及维生素类成分;该类成分抗氧化活性较强。

Objective To study liposoluble constituents and antioxidant activity of Bothriospermum tenellum.Methods The liposoluble constituents(petroleum ether extract)of Bothriospermum tenellum were detected by gas chromatography-mass spectrometry(GC-MS),and identified with reference to the data in the standard mass spectrometry library.DPPH and ABTS+free radical scavenging tests were used to detect the antioxidant activity of Bothriospermum tenellum with vitamin C as the positive control.Results total of 46 chemical constituents were detected and 22 main chemical constituents were identified.The chemical constituents with higher content included palmitic acid(26.19%),trans-13-octadecanoic acid(22.92%),tocopherol(10.03%),5,7-dimethoxycoumarin(8.80%),inositol(4.55%),etc.The 50% inhibitory concentration(IC_(50))of liposoluble constituents of the medicinal material on DPPH free radical was 208.69μg/mL,the IC50of ABTS^(+) free radical was 146.00μg/mL,and the IC_(50) of positive control was 25.80μg/mL and 21.20μg/mL,respectively.Conclusion tenellum are mainly fatty acids,fatty alcohols and vitamins,and these constituents have strong antioxidant activity.