基于应激诱导潜伏Ⅰ型单纯疱疹病毒复发的“肝郁化火”证研究进展

Research progress of Gan-Yu-Hua-Huo syndrome based on emotional stress-induced latent herpes simplex virus-1 reactivation

“肝郁化火”是中医情志致病理论的核心内容之一,广泛存在于各种相关疾病的病理过程中,是情志相关病证病机转变的关键环节。然而,由于缺乏符合中医药作用特点的动物模型,严重影响了“肝郁化火”证的生物医学基础研究,限制了“疏肝泻火”等清热中药作用机制的研究进展。在研究中发现“肝郁化火”证的颜面部火热症状的部分病理过程,与情志应激诱发潜伏Ⅰ型单纯疱疹病毒(herpes simplex virus-1,HSV-1)的激活复发引起的头面部症状有相通之处,因此提出采用情志应激诱导潜伏HSV-1激活可以建成“肝郁化火”病证结合的动物模型。该文对近年作者课题组及国内外相关文献进行了总结和分析,从情志应激导致潜伏HSV-1复发感染角度来建立“肝郁化火”的实验动物模型,揭示“肝郁化火”病理过程中的效应分子、效应靶标与机制通路,为“肝郁化火”临床常用中药的药效表征方法提供参考和依据。

Gan-Yu-Hua-Huo syndrome(Live qi stagnation transforming into fire pattern)is one of the core contents of the theory of emotional diseases in traditional Chinese medicine(TCM).It is the key link of the pathogenesis change of emotion-related diseases and widely exists in the pathological process of various related diseases.However,due to the lack of animal models in line with the characteristics of TCM syndromes,the research on biomedical basis of Gan-Yu-Hua-Huo syndrome and study of Chinese medicines for soothing liver and purging fire have been restricted seriously.This study found that the pathological process of facial fire-heat symptoms of Gan-Yu-Hua-Huo syndrome was similar to the facial symptoms due to the emotional stress-induced latent herpes simplex virus-1(HSV-1)reactivation.Therefore,this study proposed that the emotional stress-induced latent HSV-1 activation be used to establish the animal model of Gan-Yu-Hua-Huo syndrome.In this study,the state-of-art literature in the field of Gan-Yu-Hua-Huo syndrome was summarized,and the experimental animal model of Gan-Yu-Hua-Huo syndrome was established from the perspective of emotional stress-induced latent HSV-1 reactivation to reveal the active substances,potential targets and pathways related to the pathological mechanism of the syndrome.This study was expected to provide reference and basis for the pharmacodynamic characterization of commonly used Chinese medicine for Gan-Yu-Hua-Huo syndrome in clinical practice.