沉香暴食害虫黄野螟性信息素分泌源EAG和GC-MS分析

Localization of the Sex Pheromone Source in Agarwood Defoliator Heortia vitessoides Moore

为了探究黄野螟雌蛾性信息素腺体的具体部位,将雌蛾解剖为头、胸、腹、翅4部分,分别使用其挥发性气味对雄蛾进行触角电生理(EAG)检测,确定雌蛾腹部挥发物可以刺激黄野螟雄蛾产生最强的电生理响应。通过使用雌蛾腹部前、中、后3段的挥发物信号刺激雄蛾触角的电生理检测,进一步发现雌蛾腹部中段挥发物信号对雄蛾触角具有最强电生理活性,指示雌蛾腹部中段最可能是雌蛾性信息素释放的主要部位。另外,对雌蛾腹部3段结构提取挥发物的气相色谱-质谱联(GC-MS)检测和总离子图(TIC)对比,结果发现腹部中段挥发物成分中具有2个雌虫特异性成分,为该组织最强电生理活性提供了可能的物质基础。该研究结果为进一步鉴定开发黄野螟的高效性诱剂确定了成分来源的重要基础。

In order to explore the specific location of sex pheromone glands in female moths,we dissected female moths into four segments:head,thorax,abdomen and wing,and conducted electroantennogram(EAG)tests using the male antennae.Then we dissected the female abdomen into front,middle and hind segments for EAG.Finally,the three female abdominal segments were extracted by headspace solid phase micro-extraction(HS-SPME)for gas chromatography coupled with mass spectrometry(GC-MS).The abdomen was identified as the most EAG active.Further dissection confirmed the strongest response was from the middle abdominal segments.Comparative analysis for the total ion chromatograph(TIC)revealed two female EAG active abdominal segments specific peaks as candidate pheromone compounds.The female middle abdominal segments were identified as the most EAG active and there are two female EAG active abdominal segments specific peaks as candidate pheromone compounds.This provided strong basis for the identification and design of high efficiency sex pheromone lure for H.vitessoides control.