肟菌酯在斑马鱼中的生物富集效应研究

Bioaccumulation of trifloxystrobin in zebrafish(Danio rerio)

采用PRiME HLB固相萃取结合超高效液相色谱-串联质谱仪,建立了斑马鱼中肟菌酯残留的检测方法,并在此基础上研究了肟菌酯在斑马鱼中的生物富集效应。结果表明:在0.0001~0.05 mg/L范围内,肟菌酯进样质量浓度与峰面积之间具有良好的线性关系;当添加水平分别为0.0005和0.05 mg/L时,肟菌酯在水样中的平均回收率为86.6%~106%,相对标准偏差(RSD)为5.1%~8.9%;添加水平分别为0.01、0.1和10 mg/kg时,其在鱼肉中的平均回收率为70.5%~104%,RSD为1.4%~3.3%。肟菌酯在水样中的定量限(LOQ)为0.0005 mg/L,在鱼肉中的LOQ为0.01 mg/kg。利用所建立的方法,研究了在水生生物鱼类的1/10急性毒性基准剂量(ALB)值(7.15×10^(-4)mg/L)和ALB值(7.15×10^(-3)mg/L)、斑马鱼的1/100 LC_(50)值(5.10×10^(-4)和1.20×10^(-3)mg/L)以及1/10 LC_(50)值(5.10×10^(-3)和1.20×10^(-2)mg/L)剂量暴露下,肟菌酯在斑马鱼中的生物富集效应。结果表明:经不同浓度肟菌酯连续暴露192 h后,肟菌酯在斑马鱼中的生物富集系数(BCF)为113~178,具有中等生物富集效应。研究表明,生产中在使用肟菌酯时,应考虑其低浓度暴露下因生物富集作用而对水生生物引起的生态毒理学风险。

A residue analytical method of trifloxystrobin in zebrafish was established by PRiME HLB solid-phase extraction coupled with ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).Based on the established method,the bioaccumulation effect of trifloxystrobin on zebrafish was estimated.The results showed that the calibration curves for trifloxystrobin was linear in the range of 0.0001-0.05 mg/L.At fortified levels of 0.0005 and 0.05 mg/L,the average recoveries of trifloxystrobin in water ranged from 86.6%to 106%with relative standard deviations(RSDs)of 5.1%-8.9%.The average recoveries in fish were 70.5%-104%,with RSDs of 1.4%-3.3%at the fortified levels of 0.01,0.1 and 10 mg/kg.The limit of quantification(LOQ)of trifloxystrobin in water and fish was 0.0005 mg/L and 0.01 mg/kg,respectively.Based on the aquatic life benchmark(ALB)of acute toxic to fish and the LC_(50)value of zebrafish,the bioaccumulation effect of trifloxystrobin on zebrafish was studied at six exposure doses,which included the 1/10 ALB(7.15×10^(-4)mg/L),ALB(7.15×10^(-3)mg/L),the 1/100 LC_(50)(5.10×10^(-4),1.20×10^(-3)mg/L),and 1/10 LC_(50)(5.10×10^(-3),1.20×10^(-2)mg/L).After continuous exposure for 192 h,the bioconcentration factor(BCF)of trifloxystrobin ranged from 113 to 178 at different exposure doses,suggested that trifloxystrobin had a medium bioconcentration effect on zebrafish.The results indicated that in the process of application of trifloxystrobin,the ecotoxicological risk caused by the bioaccumulation effect of low concentration exposure of trifloxystrobin on aquatic species should be considered.