非洲猪瘟病毒对猪肺泡巨噬细胞mRNAs和microRNAs表达模式的影响

Effects of African swine fever virus on mRNAs and microRNAs Expression Patterns of Porcine Alveolar Macrophages

旨在探究非洲猪瘟(ASFV)病毒感染猪肺泡巨噬细胞后在体内的作用方式。以感染ASFV的猪肺泡巨噬细胞(PAMs)的mRNAs和microRNAs测序数据为基础,利用生物信息学软件对数据进行处理,得到了一批差异表达基因;利用基因功能富集和对应蛋白互作解析生物学通路;再利用mRNAs和microRNAs的互作方式确认重要的靶标关系。结果表明:ASFV改变了PAMs中1181个基因的表达量;主要调控宿主在能量代谢过程(如脂质分解、有机羟基化合物代谢、碳水化合物分解代谢、一元羧酸代谢),以及细胞周期过程(如有丝分裂细胞周期过程、细胞周期相变、细胞周期的正向调控);ASFV还引起了PAMs中25个microRNAs的差异表达,包括病毒相关的miR-27b-3p、miR-193a-3p、miR-132等;而miR-27b-3p作为表达丰度最高的差异microRNA,它与一些重要的差异基因(如PLK2、HSP90AA1等)存在着靶标关系,进而调控机体免疫和细胞周期相关通路。综上所述,ASFV感染PAMs后的作用方式包括破坏宿主在能量代谢和细胞周期方面的稳态,以及打破了机体microRNAs调节平衡;其中miR-27b-3p等小RNAs可以作为ASFV研究的靶向分子。

The aim of this study was to investigate the effect of African swine fever virus(ASFV)after infecting swine alveolar macrophages in vivo.Based on the mRNAs and microRNAs sequencing data of pig alveolar macrophages(PAMs)infected with ASFV,using bioinformatics software to process the data to obtain a number of differentially expressed genes;using gene function enrichment and corresponding protein interaction to analyze biological pathways;and using mRNAs and microRNAs interaction to confirm the important target relationships.The results showed that ASFV changed the expression level of 1181 genes in PAMs;mainly regulate the host in the energy metabolism processes(eg.,lipid decomposition,organic hydroxy compound metabolism,carbohydrate catabolism,monocarboxylic acid metabolism),and also in the cell-cycle process(eg.,mitotic cell cycle process,cell cycle phase transition,cell cycle positive regulation);ASFV also elicited the differential expression of 25 microRNAs in PAMs,including the virus-related miR-27b-3p,miR-193a-3p,and miR-132,etc.;however,miR-27b-3p,as the differential microRNA with the highest expression abundance,had a target relationship with some important differential genes(eg.,PLK2,HSP90AA1,etc.),and regulated the body′s immunity and cell cycle-related pathways.In conclusion,this study showed that the mode of action after ASFV infection with PAMs included destroying the host homeostasis through energy metabolism and cell cycle,and breaking the microRNAs regulation balance of the body;and small RNAs such as miR-27b-3p could be used as target molecules for ASFV research.