丹参酮ⅡA对人卵巢癌A2780细胞增殖、迁移和自噬的影响

Effects of TanshinoneⅡA on proliferation,migration and autophagy of human ovarian cancer A2780 cells

目的探讨丹参酮ⅡA对人卵巢癌A2780细胞增殖、迁移和自噬的影响。方法用不同浓度丹参酮ⅡA作用于卵巢癌A2780细胞24 h,CCK-8法测定不同浓度丹参酮ⅡA对A2780细胞增殖的影响,计算其IC_(50)值;依据IC_(50)值将卵巢癌A2780细胞分为4个组:0 mg/L组(即对照组)、0.3 mg/L组、0.6 mg/L组、1.2 mg/L组;应用划痕实验检测丹参酮ⅡA对A2780细胞迁移能力影响;Western Blot实验检测丹参酮ⅡA对A2780细胞凋亡相关蛋白(Bax、Bcl-2)和自噬相关蛋白(Beclin-1、LC3Ⅰ、LC3Ⅱ)表达的影响。结果CCK-8实验结果显示,丹参酮ⅡA能够抑制卵巢癌A2780细胞的增殖,且抑制作用呈现浓度依懒性,其IC_(50)值为(2.97±0.09)mg/L;划痕结果显示,与对照组相比,丹参酮ⅡA能够抑制卵巢癌A2780细胞的迁移能力,呈剂量依赖性关系;Western Blot结果显示,丹参酮ⅡA可抑制Bcl-2蛋白表达,增强Bax蛋白表达,且Bax/Bcl-2比值增高,自噬相关蛋白Beclin-1表达增加,LC3Ⅱ/LC3Ⅰ比值增高。结论丹参酮ⅡA可抑制人卵巢癌A2780细胞的增殖、迁移及促进细胞凋亡和自噬的发生。

Objective To investigate the effects of Tanshinone IIA on the proliferation,migration and autophagy of human ovarian cancer A2780 cells.Methods Ovarian cancer A2780 cells were treated with different concentrations of Tanshinone IIA for 24 h.The effect of different concentrations of Tanshinone IIA on the proliferation of A2780 cells was determined by the CCK-8 method,and the IC_(50)values were calculated.Ovarian cancer A2780 cells were divided into four groups according to the IC_(50)values:0 mg/L group(i.e.control group),0.3 mg/L group,0.6 mg/L group and 1.2 mg/L group.The effect of Tanshinone IIA on the migration ability of A2780 cells was examined by scratch assay.The effect of Tanshinone IIA on the expression of apoptosis-related proteins(Bax and Bcl-2)and autophagy-related proteins(Beclin-1,LC3Ⅰand LC3Ⅱ)was examined by Western Blotting.Results The results of CCK-8 assay showed that Tanshinone IIA inhibitted the proliferation of ovarian cancer A2780 cells,and the inhibitory effect showed concentration-dependent laziness with an IC_(50)value of(2.97±0.09)mg/L.The scratch results showed that Tanshinone IIA inhibitted the migration ability of ovarian cancer A2780 cells compared with the control group in a dose-dependent relationship.Western Blot results showed that Tanshinone IIA inhibitted the expression of Bcl-2 protein,enhanced the expression of Bax protein,and increased the ratio of Bax/Bcl-2,increased the expression of autophagy-related protein Beclin-1,and increased the ratio of LC3Ⅱ/LC3Ⅰ.Conclusion Tanshinone IIA could inhibit the proliferation and migration of human ovarian cancer A2780 cells and promoted the development of apoptosis and autophagy.