2018—2020年广西化妆品微生物污染状况分析及污染菌鉴定

Analysis of microbial contamination of cosmetics and identification of contaminated bacteria in Guangxi from 2018 to 2020

考察分析广西化妆品微生物污染状况。按《化妆品安全技术规范》(2015年版)对化妆品进行微生物学指标检验,对结果进行统计分析,通过MALDI-TOF-MS和16S rRNA测序技术等方法鉴定检出污染菌,并对污染菌株进行同源性比对分析。2018—2020年期间1 538批化妆品,合格1 521批,总合格率为98.9%。其中,菌落总数合格率99.0%,霉菌和酵母菌合格率99.9%,铜绿假单胞菌合格率99.9%,金黄色葡萄球菌、耐热大肠菌群合格率均为100.0%。微生物学指标合格率逐年上升,但仍有部分品种存在微生物污染超标情况,部分样品检出目前标准规定以外的条件致病菌(如洋葱伯克霍尔德菌复合体),应引起重视。

According to the “Technical Specification for Safety of Cosmetics”(2015 edition),the cosmetics are tested for microbial indicators.The contaminated bacteria are identified by malDI-TOF-MS and 16S rRNA sequencing technology,and the homology of the contaminated strains is compared and analyzed to investigate the microbial contamination of cosmetics in Guangxi.The results show that the homology of the same bacterial strain(Pluralibacter gergoviae) from three batches of mask of the same manufacturer is high after comparison and analysis.A total of 1 521 batches are qualified among 1 538 batches of cosmetics with the pass rate of 98.9% during 2018 to 2020.Among them,the qualified rate of aerobic bacterial count is 99.0%,the qualified rate of mold and yeast is 99.9%,the qualified rate of Pseudomonas aeruginosa is 99.9%,and the qualified rate of Staphylococcus aureus and heat-resistant coliform is 100.0%.The samples of contaminated microorganisms are mainly facial mask,skin care,toothpaste,hair care,and skin care products for infants.The unqualified samples are 10 batches of facial mask,4 batches of skin care,2 batches of toothpaste and 1 batch of hair care.The work indicates that the contaminated microorganisms are exceeded the standard in some cosmetics,and the conditioned pathogens above the standards(such as Burkholderia cepacia complex,BCC and Pluralibacter gergoviae) are detected in some samples.Although the qualified rate of microbial indicators in cosmetics is increasing year by year,microbial contamination still needs to be paid attention to.